We studied the effect of intraperitoneal and intratesticular administration of NKDTKIAKKNle-A562-572 peptide and its palmotoylated analog NKDTKIAKK-Nle-A562-572-K(Palm)A to male rats on adenylate cyclase activity in the testicular membranes in vitro and on plasma testosterone levels. Peptide NKDTKIAKK-Nle-A562-572-K(Palm)A stimulated basal adenylate cyclase activity and reduced activity of the enzyme in testicular membranes stimulated by chorionic gonadotropin. After intratesticular administration in a dose of 200 μg/kg, it significantly increased testosterone so that 1, 3 and 5 h after administration its level was increased by 74, 44 and 35%, respectively. Administered intraperitoneally in a lower dose (50 μg/kg), the peptide had little effect on testosterone level. Unmodified peptide was inactive.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
K. V. Derkach, I. V. Moyseyuk, O. V. Chistyakova, and A. O. Shpakov, Bull. Exp. Biol. Med., 155, No. 3, 339-342 (2013).
A. O. Shpakov, Tsitologiya, 51, No. 8, 637-649 (2009).
A. O. Shpakov, and E. A. Shpakova, Biomed. Khimiya, 56, No. 3, 303-318 (2010).
A. O. Shpakov, E. A. Shpakova, I. I. Tarasenko, and K. V. Derkach, Biol. Membrany, 28, No. 6, 453-462 (2011).
J. Cisowski, K. O’Callaghan, A. Kuliopulos, et al., Am. J. Pathol., 179, No. 1, 513-523 (2011).
L. Covic, M. Misra, J. Badar, et al., Nat. Med., 8, No. 10, 1161-1165 (2002).
J. Miller, A. Agarwal, A. Devi, et al., Ann. N.Y. Acad. Sci., 1180, Suppl. 1, E1-E12 (2009).
K. O’Callaghan, A. Kuliopulos, and L. Covic, J. Biol. Chem., 287, No. 16, 12 787-12 796 (2012).
D. Puett, Y. Li, G. DeMars, et al., Mol. Cell. Endocrinol., 260, No. 260-262, 126-136 (2007).
A. O. Shpakov, Int. J. Biochem. Res. Rev., 3, No. 4, 380-400 (2013).
A. O. Shpakov, I. A. Gur’yanov, L. A. Kuznetsova, et al., Neurosci. Behav. Physiol., 37, No. 7, 705-714 (2007).
A. O. Shpakov and M. N. Pertseva, Signal Transduction Research Trends, Ed. N. O. Grachevsky, New York (2007), pp. 45-93.
A. O. Shpakov, E. A. Shpakova, and K. V. Derkach, Curr. Top. Pept. Protein Res., 13, 61-73 (2012).
A. O. Shpakov, E. A. Shpakova, I. I. Tarasenko, et al., Int. J. Pept. Res. Ther., 16, No. 2, 95-105 (2010).
S. L. Tressel, G. Koukos, B. Tchernychev, et al., Methods Mol. Biol., 683, 259-275 (2011).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 158, No. 8, pp. 171-175, August, 2014
Rights and permissions
About this article
Cite this article
Derkach, K.V., Shpakova, E.A. & Shpakov, A.O. Palmitoylated Peptide 562-572 of Luteinizing Hormone Receptor Increases Testosterone Level in Male Rats. Bull Exp Biol Med 158, 209–212 (2014). https://doi.org/10.1007/s10517-014-2724-5
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-014-2724-5