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Bifidobacterium Longum Modified Recombinant HU Protein as a Vector for Nonviral Delivery of DNA to HEK293 Human Cell Culture

  • Genetics
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Bulletin of Experimental Biology and Medicine Aims and scope

Creation of effective nontoxic highly specific systems for nonviral transportation of DNA is one of the priority problems in the development of genotherapeutic methods. Chimerical recombinant proteins consisting of Antp and Tat protein cell-penetrating domains and Bifidobacterium longum DNA-binding histone-like protein HU were obtained. The resultant recombinant proteins bind to plasmid DNA in vitro and provide intracellular delivery and expression of the reporter genetic construction with GFP gene in cultured HEK293 human cells.

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Authors and Affiliations

  1. Russian State Medical University the Russian Ministry of Health Care and Social Development of the Russian Federation, Moscow, Russia

    E. V. Khokhlova, B. A. Efimov, L. I. Kafarskaia, K. A. Pavlov & A. N. Shkoporov

Authors
  1. E. V. Khokhlova
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  2. B. A. Efimov
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  3. L. I. Kafarskaia
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  4. K. A. Pavlov
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  5. A. N. Shkoporov
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Corresponding author

Correspondence to A. N. Shkoporov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 151, No. 6, pp. 663–667, June, 2011

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Khokhlova, E.V., Efimov, B.A., Kafarskaia, L.I. et al. Bifidobacterium Longum Modified Recombinant HU Protein as a Vector for Nonviral Delivery of DNA to HEK293 Human Cell Culture. Bull Exp Biol Med 151, 717–721 (2011). https://doi.org/10.1007/s10517-011-1424-7

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  • DOI: https://doi.org/10.1007/s10517-011-1424-7

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