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Role of protein kinase C in Ca2+ homeostasis disorders in cultured rat neurons during hyperstimulation of glutamate receptors

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Abstract

The primary culture of rat cerebellar neurons was used to study protein kinase C activity, intracellular variations in calcium concentration ([Ca2+]i), changes in the mitochondrial potential, and neuronal death during hyperstimulation of glutamate receptors and after 24-h incubation with phorbol ester. Prolonged exposure of neurons to glutamate (100 µM, 45 min) was followed by the development of delayed calcium dysregulation. Protein kinase C activity depended on the time of cell incubation with glutamate. Protein kinase C activity increased in response to application of glutamate for 15 min. However, protein kinase C activity decreased after 45-min exposure to glutamate and development of delayed calcium dysregulation. Protein kinase C activity was nearly undetected after 24-h preincubation of neurons with phorbol ester. Under these conditions, delayed calcium dysregulation developed more slowly and was observed in a smaller number of neurons. Neuronal death decreased to 2±1%. Our results suggest that protein kinase C plays an important role in death of neurons, which exhibit delayed calcium dysregulation during glutamate treatment.

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References

  1. A. Boldyrev, E. Bulygina, O. Gerasimova, et al., Biokhimiya, 69, No. 4, 530–536 (2004).

    Google Scholar 

  2. B. I. Khodorov, T. P. Storozhevykh, A. M. Surin, et al., Biol. Membrany, 18, No. 6, 419–430 (2001).

    Google Scholar 

  3. B. Chakravarthy, P. Morley, and J. Whitfield, Trends Neurosci., 22, 12–16 (1999).

    Article  CAS  PubMed  Google Scholar 

  4. R. Choi, Ann. N. Y. Acad. Sci., 747, 162–171 (1995).

    Google Scholar 

  5. J. Durkin, R. Tremblay, B. Chakravarthy, et al., J. Neurochem., 68, No. 4, 1400–1412 (1997).

    Article  CAS  PubMed  Google Scholar 

  6. M. Favaron, H. Manev, R. Siman, et al., Proc. Natl. Acad. Sci. USA, 87, No. 5, 1983–1987 (1990).

    Article  CAS  PubMed  Google Scholar 

  7. B. I. Khodorov, Prog. Biophys. Mol. Biol., 86, No. 2, 279–351 (2004).

    Article  CAS  PubMed  Google Scholar 

  8. J. MacDonald, S. Kotecha, W. Lu, and M. Jackson, Curr. Drugs Targets, 2, No. 2, 299–312 (2001).

    Article  CAS  Google Scholar 

  9. H. Manev, M. Favaron, A. Guidotti, and E. Costa, Mol. Pharmacol., 36, No. 1, 106–112 (1989).

    CAS  PubMed  Google Scholar 

  10. D. Mochly-Rosen and A. Gordon, FASEB J., 12, No. 1, 35–42 (1998).

    CAS  PubMed  Google Scholar 

  11. D. Nicholls and S. Budd, Physiol. Rev., 80, No. 1, 315–360 (2000).

    CAS  PubMed  Google Scholar 

  12. D. G. Nicholls and M. W. Ward, Trends Neurosci., 23, No. 1, 166–174 (2000).

    Article  CAS  PubMed  Google Scholar 

  13. Y. Nishizuka, Science, 258, 607–614 (1992).

    Article  CAS  PubMed  Google Scholar 

  14. V. G. Pinelis, M. Segal, V. Greenberger, and B. I. Khodorov, Biochem. Mol. Biol. Int., 32, No. 3, 475–482 (1994).

    CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Laboratory of Membranology, Research Center of Child Health, Moscow, Russia

    N. A. Persiyantseva, K. R. Birikh, E. A. Dvoretskova & V. G. Pinelis

  2. Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia

    B. I. Khodorov

Authors
  1. N. A. Persiyantseva
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  2. K. R. Birikh
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  3. E. A. Dvoretskova
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  4. V. G. Pinelis
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  5. B. I. Khodorov
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Correspondence to N. A. Persiyantseva.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 145, No. 5, pp. 533–537, May, 2008

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Persiyantseva, N.A., Birikh, K.R., Dvoretskova, E.A. et al. Role of protein kinase C in Ca2+ homeostasis disorders in cultured rat neurons during hyperstimulation of glutamate receptors. Bull Exp Biol Med 145, 595–599 (2008). https://doi.org/10.1007/s10517-008-0159-6

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  • DOI: https://doi.org/10.1007/s10517-008-0159-6

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