Abstract
Enterocytozoon hepatopenaei (EHP) is a fungal pathogen that causes hepatopancreatic microsporidiosis (HPM) in shrimp, resulting in significant economic losses for the shrimp culture industry. Although the impact of EHP on hormonal regulation in shrimp has been seldom examined, it remains a crucial area of study. In this paper, we analyzed the transcriptomic data of the hepatopancreas of EHP-infected and uninfected shrimp to explore the effect of EHP on hormonal regulation. Our results identified 157 differentially expressed genes (DEGs), of which 32 genes were upregulated and 125 were downregulated. Through gene ontology (GO) and KEGG analyses of hormone regulation-related DEGs, we identified eight genes, six of which were associated with the regulation of molting and growth, one with immunity, and one with osmotic pressure regulation. The up- and downregulated pathways were mostly related to molting and growth regulation, including EGFR tyrosine kinase inhibitor resistance, thyroid hormone signaling pathway, and steroid hormone biosynthesis. Ten genes were selected randomly for qRT-PCR, and results confirmed the findings of transcriptome sequencing. This study provides essential insights into the molecular mechanisms through which EHP affects hormonal regulation in shrimp and serves as a basis for further research to control HPM.
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The data that support the results of the present study are available from the corresponding author upon reasonable request.
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The authors thank the anonymous reviewers for their valuable comments and suggestions.
Funding
The research was funded by Project of Jiangsu Province “333” excellent young talent (Su Talent Office [2022] 21), the Research Funds of the Jiangsu Marine Bioresources Innovation Center, China, grant number 2022YHTZZZ04, and Yancheng Fishery High Quality Development Research Project, grant number YCSCYJ2021005.
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L.Z., S.Z., and H.S. designed the study. L.Z., S.Z.,Y.D., Y.Q., X.C., X.F., Y.H., and J.Q. analyzed the data. L.Z. and S.Z. wrote the manuscript.
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All samples and methods used in the present study were conducted in accordance with the Laboratory Animal Management Principles of China. All experimental protocols were approved by the Ethics Committee for Animal Experiments of the Jiangsu Marine Fisheries Research Institute. All shrimp handling was performed under ice anesthesia.
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Zhang, L., Zhang, S., Dou, Y. et al. Hormonal regulation changes in shrimp infected with Enterocytozoon hepatopenaei: a transcriptomic analysis. Aquacult Int (2023). https://doi.org/10.1007/s10499-023-01299-x
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DOI: https://doi.org/10.1007/s10499-023-01299-x