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Jellyfish Acromitus flagellatus (Maas) nematocyst venom-mediated biogenic synthesis of gold nanoparticles and its anti-proliferative effects

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Abstract 

The present study examines the biogenic preparation of gold nanoparticles using jellyfish Acromitus flagellatus (A. flagellatus) nematocyst venom and their anti-proliferative studies in HEK-293 (normal) and MCF-7 (cancer) cell lines. The nematocyst venom-mediated biogenic prepared gold nanoparticles were characterized using UV–Vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM). TEM micrographs showed that biosynthesized gold nanoparticles possess spherical and triangle morphology with size ranges of about 12–53 and 173–217 nm, respectively. Furthermore, the MTT assay was performed to investigate the anti-proliferative effect of nematocyst venom and nematocyst venom-mediated biogenic gold nanoparticles. The results reported that the nematocyst venom-mediated biogenic gold nanoparticles reduced the cell proliferation in cancer cells MCF-7 with an IC50 of 45.65 μg mL−1 and 18.24 μg mL−1 compared to normal cells (HEK-293) at 48 h. These findings indicated that gold nanoparticles with venom residue exert their anti-proliferative action on the MCF-7 cell line.

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Acknowledgements

The authors thank the management of the Sathyabama Institute of Science and Technology (SIST) for its strong support to carry out research activities.

Funding

The Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, New Delhi, provided the financial support under the scheme of Aquaculture and Marine Biotechnology (SAN & KG) (BT/PR15485/AAQ/3/754/2015).

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Authors and Affiliations

  1. Centre for Ocean Research, Sathyabama Institute of Science and Technology, Chennai, 600119, India

    S. Amreen Nisa, K. Govindaraju & R. Vasantharaja

  2. Centre for Agricultural Nanotechnology, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    M. Kannan & K. Raja

Authors
  1. S. Amreen Nisa
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  2. K. Govindaraju
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  3. R. Vasantharaja
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  4. M. Kannan
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  5. K. Raja
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Contributions

S. Amreen Nisa: Methodology, Investigation, Validation; K. Govindaraju: Conceptualization, Project administration, Writing - review & editing. R. Vasantharaja: Formal analysis, Investigation; Writing - review & editing; M. Kannan: Formal analysis; D. K. Raja: Validation, Formal analysis. All authors reviewed the manuscript.

Corresponding author

Correspondence to K. Govindaraju.

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The authors declare no competing interests.

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Handling Editor: Raja Sudhakaran

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Highlights

• Jellyfish Acromitus flagellatus nematocyst venom-mediated biogenic gold nanoparticles.

• Physicochemical characterization of biogenic gold nanoparticles was performed.

• TEM micrographs showed spherical and triangle morphology of biogenic gold nanoparticles.

• Anti-proliferative studies in HEK-293 (normal) and MCF-7 (cancer) cell lines.

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Nisa, S.A., Govindaraju, K., Vasantharaja, R. et al. Jellyfish Acromitus flagellatus (Maas) nematocyst venom-mediated biogenic synthesis of gold nanoparticles and its anti-proliferative effects. Aquacult Int 31, 2235–2244 (2023). https://doi.org/10.1007/s10499-023-01081-z

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  • DOI: https://doi.org/10.1007/s10499-023-01081-z

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