Abstract
Macrobrachium rosenbergii is a typical aquatic organism with reversible gonadal development that is regulated by gene expression. The role of transcription factors in gonadal in adult shrimps remains unclear in M. rosenbergii. In this study, we sequenced the transcriptomes of adult shrimp testes, ovaries, and androgenic glands using second-generation sequencing. In total, 24,007 genes were identified and 9,199 differentially expressed genes (DEGs) were identified by pairwise comparison. There were 272 differentially expressed transcription factors (TFs); 107, 152, and 13 differentially expressed TFs were identified in the testes, ovaries, and androgenic glands by three pairwise comparisons, respectively. GO and KEGG analyses of the TFs and DEGs involved in the MAPK signaling and transcriptional regulation pathways and play key roles in the cell cycle of the testes, whereas involved in the thyroid hormone signaling pathway and neuroactive ligand–receptor interaction play important roles in the ovary. We determined the existence of networks comprising important TFs related to sex development in adult M. rosenbergii gonads. The key TFs were Piwi (expressed only in the testes and ovaries) and Argonaute 3 (expressed only in the ovaries), which might be involved in the regulation of testes and ovary development.
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Data availability
All data generated in this study were deposited in the Gene Expression Omnibus (GEO) with the accession code GSE171823.
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This work was supported by the Zhejiang Provincial Natural Science Foundation under Grant number LY21C190001.
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Jindong Ren: methodology, samples collection, data analysis, writing original draft, funding acquisition, project administration. Rong Na: writing—review and editing. Honglin Chen: methodology. Bao Lou: project administration, supervision. Baolong Niu: methodology, supervision.
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Ren, J., Na, R., Chen, H. et al. RNA sequencing and functional analysis of adult gonadal tissue to identify candidate key genes in Macrobrachium rosenbergii sex development. Aquacult Int 29, 2805–2821 (2021). https://doi.org/10.1007/s10499-021-00780-9
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DOI: https://doi.org/10.1007/s10499-021-00780-9