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Cryopreservation of scaly carp (Cyprinus carpio) sperm: effect of different cryoprotectant concentrations on post-thaw motility, fertilization and hatching success of embryos

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Abstract

The aim of the present study was to determine the effect of various cryoprotectants on post-thaw sperm quality and fertilizing capacity of cryopreserved scaly carp (Cyprinus carpio) semen. The present study focused on freezing of scaly carp sperm utilizing a practical and inexpensive protocol for aquaculture. Semen was diluted with Kurokura’s extender composing 3.6 g/l NaCl, 10 g/l KCl, 0.22 g/l CaCl2, 0.08 g/l MgCl2 and 0.2 g/l NaHCO3. The extender contained three different cryoprotectants (DMSO, DMA and egg yolk) at ratios of 5, 10 and 15 %. Semen was placed into 0.25-ml straws and exposed to liquid nitrogen vapor (−120 °C) using an insulated box with an adjustable tray for 10 min and then plunged into liquid nitrogen (−196 °C) tank. The thawing process was performed in a water bath at 40 °C for 10 s. The results indicated that type of cryoprotectants and their concentrations are rather effective in scaly carp sperm cryopreservation on post-thaw sperm quality, while they are very important in order to obtain high fertilization rates. The highest fertilization rate was determined as 96.4 ± 0.15 % with 15 % egg yolk, while the highest hatching rate was determined as 99.3 ± 0.80 with 15 % DMA. In conclusion, the applied cryopreservation method for scaly carp sperm is suitable to fertilize high amounts of eggs.

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Acknowledgments

This research was funded by a grant from Scientific Research Institute of Mustafa Kemal University (MKU-08-E-0206). The authors would like to thank the staff of the State Hydraulic Works (SHW) Fish Production Station in Adana (Turkey) for their technical assistance. Also, the valuable comments and suggestions from anonymous reviewers are deeply thanked.

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Authors and Affiliations

  1. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, University of Mustafa Kemal, Antakya, Hatay, Turkey

    İlker Yavaş & Cengiz Yıldız

  2. Department of Aquaculture, Faculty of Marine Science and Technology, University of Mustafa Kemal, Iskenderun, Hatay, Turkey

    Yusuf Bozkurt

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  1. İlker Yavaş
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  2. Yusuf Bozkurt
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  3. Cengiz Yıldız
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Correspondence to Yusuf Bozkurt.

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Yavaş, İ., Bozkurt, Y. & Yıldız, C. Cryopreservation of scaly carp (Cyprinus carpio) sperm: effect of different cryoprotectant concentrations on post-thaw motility, fertilization and hatching success of embryos. Aquacult Int 22, 141–148 (2014). https://doi.org/10.1007/s10499-013-9698-6

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  • DOI: https://doi.org/10.1007/s10499-013-9698-6

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