Abstract
The spermatozoa of oviparous fish, such as feral carp (Cyprinus carpio), are immotile in the presence of semen plasma or isotonic solutions, and to obtain good motility, they must be diluted with suitable medium. The objective of this study was to identify the best activating solution for feral carp sperm. Sperm motilities were compared in the new activating solution (a): (50 mM NaCl, 30 mM KCl, 30 mM Tris, pH = 8.5) and activating solution (b): (50 mM NaCl, 40 mM KCl, 30 mM Tris, pH = 8.5) based on effect of pH with everyone of Na+ and K+ ions versus four other activating solutions Billard’s saline solution, Poupard’s saline solution, distilled water and hatchery water that is routinely used for extending carp semen. Our results showed that maximum total motility period and percentage of motile sperm were seen in selected saline solution (a). The present study describes an activating solution that prolongs feral carp sperm motility.
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Acknowledgments
We would like to thank the staff at carp reproduction center of Sijoval (Golestan, Iran) for supplying of fish and Mr Rezaei and Zolfaghari for their kind help during the experiments.
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Bastami, K.D., Imanpour, M.R. & Hoseinifar, S.H. Sperm of feral carp Cyprinus carpio: optimization of activation solution. Aquacult Int 18, 771–776 (2010). https://doi.org/10.1007/s10499-009-9299-6
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DOI: https://doi.org/10.1007/s10499-009-9299-6