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Genome analysis of probiotic bacteria for antibiotic resistance genes

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Abstract

To date, probiotic bacteria are used in the diet and have various clinical applications. There are reports of antibiotic resistance genes in these bacteria that can transfer to other commensal and pathogenic bacteria. The aim of this study was to use whole-genome sequence analysis to identify antibiotic resistance genes in a group of bacterial with probiotic properties. Also, this study followed existing issues about the importance and presence of antibiotic resistance genes in these bacteria and the dangers that may affect human health in the future. In the current study, a collection of 126 complete probiotic bacterial genomes was analyzed for antibiotic resistance genes. The results of the current study showed that there are various resistance genes in these bacteria that some of them are transferable to other bacteria. The tet(W) tetracycline resistance gene was more than other antibiotic resistance genes in these bacteria and this gene was found in Bifidobacterium and Lactobacillus. In our study, the most numbers of antibiotic resistance genes were transferred with mobile genetic elements. We propose that probiotic companies before the use of a micro-organism as a probiotic, perform an antibiotic susceptibility testing for a large number of antibiotics. Also, they perform analysis of complete genome sequence for prediction of antibiotic resistance genes.

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The author thanks of Zoonotic Diseases Research Center Yazd, Iran for support.

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  1. Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Mehdi Fatahi-Bafghi

  2. Zoonotic Diseases Research Center, Department of Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Mehdi Fatahi-Bafghi & Sara Naseri

  3. International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Ali Alizehi

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  1. Mehdi Fatahi-Bafghi
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MF-B participated in the design of the study, data analysis and the draft the manuscript, SN carried out data analysis, AA participated in data analysis.

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Fatahi-Bafghi, M., Naseri, S. & Alizehi, A. Genome analysis of probiotic bacteria for antibiotic resistance genes. Antonie van Leeuwenhoek 115, 375–389 (2022). https://doi.org/10.1007/s10482-021-01703-7

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