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Jiella mangrovi sp. nov., a novel endophytic bacterium isolated from leaf of Rhizophora stylosa

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Abstract

A Gram-stain negative, non-motile, brilliant yellow and non-spore forming, coccoid- or short rod-shaped bacterium, designated strain KSK16Y-1T, was isolated from surface-sterilised leaf of Rhizophora stylosa collected from Shankou Mangrove Nature Reserve, Guangxi Zhuang Autonomous Region, China. Genome of strain KSK16Y-1T is 4.93 Mb with 68.1% DNA G + C content and encoded 4359 predicted proteins, 4 rRNAs, 45 tRNAs and 4 ncRNA. Comparative 16S rRNA gene sequence analysis showed that the strain KSK16Y-1T has 98.1%, 97.9% and 96.9% 16S rRNA gene similarities with Jiella aquimaris JCM 30119T, J. endophytica CBS5Q-3T and J. pacifica 40Bstr34T, respectively. Whole-genome comparisons between strain KSK16Y-1T and J. aquimaris 22II-16-19i, J. endophytica CBS5Q-3T, J. pacifica 40Bstr34T, using average nucleotide identity (ANI) values (< 82.0%) and digital DNA-DNA hybridization (dDDH) values (< 25.1%), confirmed low genome relatedness. Strain KSK16Y-1T grew at 20–30 °C (optimum, 30 °C), pH 6.0–11.0 (optimum, pH 6.0–7.0) and with 0–10% (w/v) NaCl (optimum, 0–2%). Cell wall contained meso-diaminopimelic acid and the major fatty acid was C18:1ω7c. The polar lipid profile consists of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, one unknown phospholipid, one unknown aminolipid, one unknown aminophospholipid and four unidentified lipids. The predominant respiratory quinone is ubiquinone-10 (Q-10). The polyphasic characterization indicated that strain KSK16Y-1T represents a novel Jiella species. The name Jiella mangrovi sp. nov., type strain KSK16Y-1T (= CGMCC 1.18745T = JCM 34332T) is proposed.

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Data availability statements

All data generated or analysed during this study are included in this published article, its supplementary information files and GenBank/EMBL/DDBJ.

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Funding

This research was supported by the National Natural Sciences Foundation of China (NSFC, Grant no. 81960642) and Guizhou Provincial Science and Technology Foundation (No. Qian Ke He Jichu [2019]1347).

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Ming-Sheng Chen, Xiao-Rui Yan and Li Tuo collected plant samples, isolated strains and preliminary identification. Ming-Sheng Chen performed experiments and wrote the manuscript. Fei-Na Li performed genome and chemotaxonomic analyses. Ming-Sheng Chen, Xiao-Hui Chen, Zi-Hao Huang and Xiao-Rui Yan performed initial physiological and morphological analyses of the strain. Li Tuo conceived and designed the experiments and approved the final manuscript.

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Correspondence to Li Tuo.

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Chen, MS., Li, FN., Chen, XH. et al. Jiella mangrovi sp. nov., a novel endophytic bacterium isolated from leaf of Rhizophora stylosa. Antonie van Leeuwenhoek 114, 1633–1645 (2021). https://doi.org/10.1007/s10482-021-01629-0

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