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Streptomyces monticola sp. nov., a novel actinomycete isolated from soil

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Abstract

A novel actinobacterium, designated strain NEAU-GS4T, was isolated from soil collected from Mount Song and characterised using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-GS4T belongs to the genus Streptomyces, being closely related to Streptomyces spectabilis JCM 4308T (98.8%), Streptomyces sclerotialus DSM 43032T (98.3%) and Streptomyces lasiicapitis 3H-HV17(2)T (98.0%). A multilocus sequence analysis based on five house-keeping genes (atpD, gyrB, rpoB, recA and trpB) also indicated that strain NEAU-GS4T should be assigned to the genus Streptomyces. The major menaquinones were identified as MK-9(H6), MK-9(H8) and MK-9(H4). The polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositolmannosides and an unidentified phospholipid. The major fatty acids were identified as anteiso-C15:0, iso-C16:0 and anteiso-C17:0. Moreover, DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-GS4T can be clearly differentiated from its closely related species of the genus Streptomyces. Therefore, it is concluded that strain NEAU-GS4T represents a novel species of the genus of Streptomyces, for which the name Streptomyces monticola sp. nov. is proposed. The type stain is NEAU-GS4T (=CGMCC 4.7467T = DSM 105116T).

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Acknowledgements

This work was supported in part by grants from the National Natural Science Foundation of China (No. 31572070). We are grateful to Professor Aharon Oren for helpful advice on the specific epithet.

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Dongmei Li, Liyuan Han, Junwei Zhao, Hanxun Ju, Shanwen Jiang, Xiaowei Guo, Xiangjing Wang & Wensheng Xiang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Wensheng Xiang

Authors
  1. Dongmei Li
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  2. Liyuan Han
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  3. Junwei Zhao
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  4. Hanxun Ju
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  5. Shanwen Jiang
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  6. Xiaowei Guo
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  8. Wensheng Xiang
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Contributions

DL performed the laboratory experiments, analyzed the data, and drafted the manuscript. LH contributed to the biochemical characterisation. JZ contributed to the polyphasic taxonomy. HJ contributed to the morphological analyzes. SJ contributed to the fatty acids determination. XG participated in the discussions of each section of experiments. XW and WX designed the experiments and revised the manuscript.

Corresponding authors

Correspondence to Xiangjing Wang or Wensheng Xiang.

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This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

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Li, D., Han, L., Zhao, J. et al. Streptomyces monticola sp. nov., a novel actinomycete isolated from soil. Antonie van Leeuwenhoek 112, 451–460 (2019). https://doi.org/10.1007/s10482-018-1177-7

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  • DOI: https://doi.org/10.1007/s10482-018-1177-7

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