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Microbial community changes during a toxic cyanobacterial bloom in an alkaline Hungarian lake

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Abstract

The Carpathian Basin is a lowland plain located mainly in Hungary. Due to the nature of the bedrock, alluvial deposits, and a bowl shape, many lakes and ponds of the area are characterized by high alkalinity. In this study, we characterized temporal changes in eukaryal and bacterial community dynamics with high throughput sequencing and relate the changes to environmental conditions in Lake Velence located in Fejér county, Hungary. The sampled Lake Velence microbial populations (algal and bacterial) were analyzed to identify potential correlations with other community members and environmental parameters at six timepoints over 6 weeks in the Spring of 2012. Correlations between community members suggest a positive relationship between certain algal and bacterial populations (e.g. Chlamydomondaceae with Actinobacteria and Acidobacteria), while other correlations allude to changes in these relationships over time. During the study, high nitrogen availability may have favored non-nitrogen fixing cyanobacteria, such as the toxin-producing Microcystis aeruginosa, and the eutrophic effect may have been exacerbated by high phosphorus availability as well as the high calcium and magnesium content of the Carpathian Basin bedrock, potentially fostering exopolymer production and cell aggregation. Cyanobacterial bloom formation could have a negative environmental impact on other community members and potentially affect overall water quality as well as recreational activities. To our knowledge, this is the first prediction for relationships between photoautotrophic eukaryotes and bacteria from an alkaline, Hungarian lake.

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Acknowledgements

The authors wish to thank Katalin Barkács and Laura Jurecska (Cooperative Research Centre for Environmental Sciences, Budapest, Hungary) for the chemical analysis of water samples. We would also like to thank Frank W. Schneider Agriculture Specialist, for U.S. Customs and Border Protection for his assistance and Dr. Bharath Prithiviraj with the Plant Biology Division at the Samuel Roberts Noble Foundation Inc. for his bioinformatics expertise. Thanks are also due to Devon Andor Bell, Susi Szeremy, Ajna Akantisz, Hugh MacMurray, and Shane Mortensen for sampling assistance.

Funding

Support was provided by the US Department of Energy-Advancements in Sustainable Algal Production (ASAP) program under contract DE-EE0005993. T.S.A. Bell was also partially supported by the NSF IGERT Program in Geobiological Systems (DGE 0654336). T. Felföldi was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. Additional support was provided by the National Research, Development, and Innovation Office of Hungary (Grant Number K 116275). G. Vasas was supported by the National Research, Development, and Innovation Office of Hungary (NKFIH-119647).

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Center for Biofilm Engineering, Montana State University, Bozeman, MT, 59717, USA

    Tisza A. S. Bell, Emel Sen-Kilic, Matthew W. Fields & Brent M. Peyton

  2. Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA

    Tisza A. S. Bell & Matthew W. Fields

  3. Department of Microbiology, Eötvös Loránd University, Budapest, Hungary

    Tamás Felföldi

  4. Energy Research Institute, Montana State University, Bozeman, MT, USA

    Matthew W. Fields & Brent M. Peyton

  5. Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, USA

    Emel Sen-Kilic & Brent M. Peyton

  6. Department of Botany, University of Debrecen, Debrecen, Hungary

    Gabor Vasas

  7. Genome Core and Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, USA

    Tisza A. S. Bell

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  1. Tisza A. S. Bell
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Bell, T.A.S., Sen-Kilic, E., Felföldi, T. et al. Microbial community changes during a toxic cyanobacterial bloom in an alkaline Hungarian lake. Antonie van Leeuwenhoek 111, 2425–2440 (2018). https://doi.org/10.1007/s10482-018-1132-7

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