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Streptomyces lonarensis sp. nov., isolated from Lonar Lake, a meteorite salt water lake in India

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Abstract

A novel alkaliphilic actinomycete, strain NCL716T, was isolated from a soil sample collected from the vicinity of Lonar Lake, an alkaline salt water meteorite lake in Buldhana district of Maharashtra State in India. The strain was characterised using a polyphasic taxonomic approach which confirmed that it belongs to the genus Streptomyces. Growth was observed over a pH range of 7–11 at 28 °C. The cell wall was found to contain ll-diaminopimelic acid and traces of meso-diaminopimelic acid. The major fatty acid components were identified as iso-C16:0 (46.8 %), C17:1 (12.4 %), anteiso-C15:0 (5.1 %) and anteiso-C17:1 (4.8 %). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The major menaquinones were determined to be MK-9 (H6) (70.3 %), MK-9 (H4) (15.5 %) and MK-9 (H8) (7.2 %). The G+C content of the DNA of the type strain was determined to be 71.4 mol  %. The 16S rRNA gene sequence has been deposited in GenBank with accession number FJ919811. Although the 16S rRNA gene sequence analysis revealed that strain NCL716T shares >99 % similarity with that of Streptomyces bohaiensis strain 11A07T, DNA–DNA hybridization revealed only 33.2 ± 3.0 % relatedness between them. Moreover, these two strains can be readily distinguished by some distinct phenotypic characteristics. Hence, on the basis of phenotypic and genetic analyses, it is proposed that strain NCL716T represents a novel species of the genus Streptomyces, for which the name Streptomyces lonarensis sp. nov., is proposed. The type strain is NCL 716T (=DSM 42084T = MTCC 11708T = KCTC 39684T).

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Acknowledgments

Trupti K. Sharma acknowledges the Council of Scientific and Industrial Research (CSIR), India, for the Senior Research Fellowship.

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Authors and Affiliations

  1. Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, 411008, India

    Trupti K. Sharma, Vidhya K. Shinde, Meenakshi V. Rele & Lalitha Sunil Kumar

  2. NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune, 411008, India

    Rahul Mawlankar, Vidya V. Sonalkar & Syed G. Dastager

  3. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, China

    Jing Zhan & Wen-Jun Li

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  1. Trupti K. Sharma
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Sharma, T.K., Mawlankar, R., Sonalkar, V.V. et al. Streptomyces lonarensis sp. nov., isolated from Lonar Lake, a meteorite salt water lake in India. Antonie van Leeuwenhoek 109, 225–235 (2016). https://doi.org/10.1007/s10482-015-0626-9

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