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Streptomyces calidiresistens sp. nov., isolated from a hot spring sediment

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Abstract

A Streptomyces-like actinomycete strain, designated as YIM 78087T, was isolated from a sediment sample collected from Hehua hot spring in Tengchong, Yunnan province, south-west China. The taxonomic position of strain YIM 78087T was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 78087T belongs to the genus Streptomyces and is closely related to Streptomyces fimbriatus DSM 40942T, Streptomyces marinus DSM 41968T and Streptomyces qinglanensis DSM 42035T (97.18, 97.05 and 97.1 % similarity, respectively). Combined with the low values of DNA–DNA hybridization between strain YIM 78087T and its closest neighbours, these analyses indicated that this new isolate represents a different genomic species in the genus Streptomyces. The predominant menaquinones of strain YIM 78087T were identified as MK-9 (H4) and MK-9 (H6). The major fatty acids were identified as anteiso-C15:0 (28.4 %), anteiso-C17:0 (23.0 %) and iso-C16:0 (15.1 %). The whole-cell hydrolysates found to contain glucose, mannose and ribose. The DNA G+C content was determined to be 73.0 mol%. Based on the comparative analysis of phenotypic and genotypic characteristics, it is proposed that strain YIM 78087T represents a novel species of the genus Streptomyces, for which the name Streptomyces calidiresistens sp. nov., is proposed. The type strain is YIM 78087T (=BCRC 16955T=DSM 42108T=JCM 19629T).

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Acknowledgments

The authors are grateful to Prof. Dr. Hans-Peter Klenk (DSMZ, Germany) for his kind providing of reference type strains. This research was supported by Key Project of International Cooperation of Ministry of Science & Technology (MOST) (No. 2013DFA31980), National Science Foundation Grant (OISE-0968421), Natural Science Foundation of China (Nos. 31070007 and 31372545), Key Project of Yunnan Provincial Natural Science Foundation (2013FA004), Scientific Research Fund of Xinxiang Medical University (2013QN126) and Research Project of Education Department of Henan Province of China (2011A180025).

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Authors and Affiliations

  1. College of Fisheries, Henan Normal University, Xinxiang, 453007, People’s Republic of China

    Yan-Yan Duan, Yi Zhang & Guo-Xing Nie

  2. College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, People’s Republic of China

    Hong Ming

  3. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Yan-Yan Duan, Hong Ming, Lei Dong, Yi-Rui Yin, En-Min Zhou, Lan Liu & Wen-Jun Li

  4. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Wen-Jun Li

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  1. Yan-Yan Duan
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Correspondence to Guo-Xing Nie or Wen-Jun Li.

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Yan-Yan Duan and Hong Ming have contributed equally to this work.

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Duan, YY., Ming, H., Dong, L. et al. Streptomyces calidiresistens sp. nov., isolated from a hot spring sediment. Antonie van Leeuwenhoek 106, 189–196 (2014). https://doi.org/10.1007/s10482-014-0180-x

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