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Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora

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Abstract

A novel actinomycete, designated strain NEAU-GRX11T, was isolated from muddy soil collected from a stream of Jinlong Mountain in Harbin, north China. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. The 16S rRNA gene sequence of strain NEAU-GRX11T showed highest similarity to Micromonospora zamorensis CR38T (99.2 %), Micromonospora saelicesensis Lupac 09T (99.0 %), Micromonospora chokoriensis 2-19/6T (98.7 %), Micromonospora coxensis 2-30-b/28T (98.5 %), Micromonospora aurantiaca ATCC 27029T (98.4 %) and Micromonospora lupini lupac 14NT (98.3 %). Phylogenetic analysis based on the 16S rRNA gene and gyrB gene demonstrated that strain NEAU-GRX11T was a member of the genus Micromonospora and supported the closest phylogenetic relationship to M. zamorensis CR38T, M. saelicesensis Lupac 09T, M. chokoriensis 2-19/6T and M. lupini lupac 14NT. A combination of DNA–DNA hybridization and some phenotypic characteristics indicated that the novel strain could be readily distinguished from these closest phylogenetic relatives. Therefore, it is proposed that NEAU-GRX11T represents a novel species of the genus Micromonospora, for which the name Micromonospora jinlongensis sp. nov. is proposed. The type strain is NEAU-GRX11T (=CGMCC 4.7103T=DSM 45876T).

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Acknowledgments

This work was supported in part by grants from the Opening Fund of Key Laboratory of Soybean Biology in Chinese Ministry of Education (No. SB12B04), the National Key Project for Basic Research (No. 2010CB126102), the National Outstanding Youth Foundation (No. 31225024), the Special Foundation for Scientific and Technological Innovation Research of Harbin (No. 2011RFXXN038) and the Natural Science Foundation of Heilongjiang Province (No. C201029).

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Authors and Affiliations

  1. School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Ruixia Gao, Chongxi Liu, Junwei Zhao, Feiyu Jia, Chao Yu & Wensheng Xiang

  2. Key Laboratory of Microbial Drug Engineering of Heilongjiang Provincial Education Committee, Harbin, 150030, People’s Republic of China

    Lingyu Yang & Xiangjing Wang

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  1. Ruixia Gao
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Ruixia Gao and Chongxi Liu contributed equally to this work.

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Gao, R., Liu, C., Zhao, J. et al. Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora . Antonie van Leeuwenhoek 105, 307–315 (2014). https://doi.org/10.1007/s10482-013-0074-3

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