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Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.

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Abstract

Endophytic actinobacteria isolated from Artemisia annua were characterized and evaluated for their bioactivities. A total of 228 isolates representing at least 19 different genera of actinobacteria were obtained and several of them seemed to be novel taxa. An evaluation of antimicrobial activity showed that more isolates possessed activity towards plant pathogens than activity against other pathogenic bacteria or yeasts. High frequencies of PCR amplification were obtained for type I polyketide synthases (PKS-I, 21.1%), type II polyketide synthases (PKS-II, 45.2%) and nonribosomal peptide synthetases (NRPS, 32.5%). The results of herbicidal activity screening indicated that 19 out of 117 samples of fermentation broths completely inhibited the germination of Echinochloa crusgalli. This study indicated that endophytic actinobacteria associated with A. annua are abundant and have potentially beneficial and diverse bioactivities which should be pursued for their biotechnical promise.

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Acknowledgments

We are grateful to Prof. Shao-Ping Li (Yunnan Agricultural Academy) for providing the Artemisia annua seeds (F38#). This research was supported by the National Basic Research Program of China (No. 2010CB833801), National Natural Science Foundation of China (No. U0932601) and Yunnan Provincial Natural Science Foundation (Nos. 2009AC017, 2009DA002).

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Authors and Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Jie Li, Guo-Zhen Zhao, Hai-Yu Huang, Wen-Yong Zhu, Li-Xing Zhao, Li-Hua Xu & Wen-Jun Li

  2. Key Laboratory of Marine Bio-resources Sustainable Utilization CAS, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, People’s Republic of China

    Jie Li & Si Zhang

  3. Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Xuzhou Normal University, Xuzhou, 221116, Jiangsu, People’s Republic of China

    Sheng Qin

  4. Department of Plant Sciences, Montana State University, Bozeman, MT, 59717, USA

    Gary Strobel

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  1. Jie Li
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  2. Guo-Zhen Zhao
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  9. Wen-Jun Li
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  10. Gary Strobel
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Correspondence to Wen-Jun Li.

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Li, J., Zhao, GZ., Huang, HY. et al. Isolation and characterization of culturable endophytic actinobacteria associated with Artemisia annua L.. Antonie van Leeuwenhoek 101, 515–527 (2012). https://doi.org/10.1007/s10482-011-9661-3

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