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Jeotgalibacillus soli sp. nov., isolated from non-saline forest soil, and emended description of the genus Jeotgalibacillus

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Abstract

A Gram-stain-positive, endospore-forming, motile, catalase- and oxidase-positive, aerobic, rod-shaped bacterium, designated strain JSM 081008T, was isolated from non-saline forest soil in China. Strain JSM 081008T was able to grow with 0–20% (w/v) NaCl, at pH 6.0–10.5 and at 10–45°C; optimum growth was observed with 2–5% (w/v) NaCl, at pH 7.0–8.0 and at 30–35°C. The peptidoglycan type was A1α linked directly through l-Lys. The major cellular fatty acids (>10% of the total) were anteiso-C15:0, iso-C15:0, anteiso-C17:0 and C16:0. The predominant respiratory quinone was menaquinone 7 and the genomic DNA G + C content of the strain was 42.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 081008T should be assigned to the genus Jeotgalibacillus and was related most closely to the type strains of Jeotgalibacillus alimentarius (sequence similarity 99.4%) and Jeotgalibacillus salarius (97.0%), followed by Jeotgalibacillus campisalis (95.4%) and Jeotgalibacillus marinus (95.2%). The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the view that strain JSM 081008T represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus soli sp. nov. is proposed. The type strain is JSM 081008T (=DSM 22174T = KCTC 13528T). An emended description of the genus Jeotgalibacillus is also presented.

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Acknowledgements

This work was supported by grants from the National Basic Research Program of China (2010CB833800), National Natural Science Foundation of China (NSFC) (30970007), Jishou University (09JDY022) and International Cooperation Research Program of Yunnan Province (2009AC017). We are grateful to Mr Ke Huang for his excellent technical assistance.

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Authors and Affiliations

  1. College of Biology and Environmental Sciences, Jishou University, 416000, Jishou, People’s Republic of China

    Yi-Guang Chen, De-Jiao Peng, Qi-Hui Chen, Da-Chun Zhang & Qing-Zhong Peng

  2. The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People’s Republic of China

    Yi-Guang Chen, Shu-Kun Tang & Wen-Jun Li

  3. Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People’s Republic of China

    Yi-Guang Chen, Shu-Kun Tang & Wen-Jun Li

  4. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, 100050, Beijing, People’s Republic of China

    Yu-Qin Zhang

  5. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, People’s Republic of China

    Wen-Jun Li

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  1. Yi-Guang Chen
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  2. De-Jiao Peng
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Corresponding authors

Correspondence to Yi-Guang Chen or Wen-Jun Li.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 081008T is FJ527421.

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Chen, YG., Peng, DJ., Chen, QH. et al. Jeotgalibacillus soli sp. nov., isolated from non-saline forest soil, and emended description of the genus Jeotgalibacillus. Antonie van Leeuwenhoek 98, 415–421 (2010). https://doi.org/10.1007/s10482-010-9455-z

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