Abstract
Pongamia pinnata an oil yielding leguminous tree is an economically important plant due to its multifunctional characteristics. The objective of the present study was to investigate the electrophoretic spectra sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of total soluble seed proteins under reduced conditions (5 mM β-mercaptoethanol) in immatured [135 days after flowering (DAF)] and matured stages (350 DAF) for 10 tagged candidate plus trees (CPTs) of P. pinnata from North Guwahati. SDS-PAGE analysis revealed presence of ~15–20 unique polypeptide fragments of molecular size ranging from 14 to 150 kDa in both stages of seed development but no variations was observed at a particular stage among 10 CPTs. However, polypeptide banding pattern of immatured and fully ripened matured seeds showed differences in expression pattern of three main polypeptide bands (MW 50, 18 and 14 kDa) for all the 10 CPTs studied. Globulins may be considered to be the main seed storage proteins in Pongamia that includes legumins and vicilins.
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References
Aluko RE, McIntosh T (2004) Electrophoretic and functional properties of mustard seed meals and protein concentrates. J Am Oil Chem Soc 81:679–683
Beckstrom-Sternberg S (1989) Two-dimensional gel electrophoresis as a taxonomic tool: evidence from the Centrospermae. Biochem Syst Ecol 17:573–582
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Casey R, Domoney C (1999) Pea globulins. In: Shewry PR, Casey R (eds) Seed proteins. Kluwer Academic Publishers, Dordrecht, pp 171–208
Casey R, Domoney C, Smith AM (1993) Biochemistry and molecular biology of seed products. In: Casey R, Davies DR (eds) Peas: genetics, molecular biology and biotechnology. CAB International, Wallingford, pp 121–163
Celis J, Bravo R (1984) Two dimensional gel electrophoresis of proteins: methods and applications. Academic Press, New York
Cooke RJ (1999) Modern methods for cultivar verification and the transgenic plant challenge. Seed Sci Technol 27:669–680
El Naggar SM (2001) Implication of seed proteins in Brassicacea systematics. Biol Plant 44:547–553
Ghafoor A, Arshad M (2008) Seed protein profiling of Pisum sativum L., germplasm using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) for investigation of biodiversity. Pak J Bot 40:2315–2321
Kesari V, Krishnamachari A, Rangan L (2008) Systematic characterization and seed oil analysis in candidate plus trees of biodiesel plant, Pongamia pinnata (L.). Ann Appl Biol 152:397–404
Kesari V, Das A, Rangan L (2009) Physico-chemical characterization and microbial assay from seed oil of Pongamia pinnata, potential biofuel crop. Biomass Bioenergy 33:1724–1728
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680–685
Lqari H, Pedroche J, Girón-Calle J, Vioque J, Millán F (2004) Purification and partial characterization of storage proteins in Lupinus angustifolius seeds. Grasas y Aceites 55:364–369
Mukta N, Sreevalli Y (2010) Propagation techniques, evaluation and improvement of the biodiesel plant, Pongamia pinnata (L.) Pierr—a review. Ind Crop Prod 31:1–12
NOVOD 2010. 5th R&D report on tree borne oilseeds. NOVOD Board India. http://www.novodboard.com/5th%20RandD-Report.pdf
Peumans WJ, Van Damme EJM (1999) Seed lectins. In: Shewry PR, Casey R (eds) Seed proteins. Kluwer Academic Publisher, Dordrecht, pp 657–683
Prakash V, Rao NMS (1986) Physical chemical properties of oil seed proteins. CRC Crit Rev Biochem 20:265–363
Rodríguez de DJ, Romero-García J, Rodríguez-García R, Sánchez JLA (2002) Characterization of proteins from sunflower leaves and seeds: relationship of biomass and seed yield. In: Janicks J, Whipkey A (eds) Trends in new crops and new uses. ASHS Press, Alexandria, pp 143–149
Santos CN, Ferreira RB, Teixeira AN (1997) Seed proteins of Lupinus mutabilis. J Agric Food Chem 45:3821–3825
Scott PT, Pregelj L, Chen N, Hadler JS, Djordjevic MA, Gresshoff PM (2008) Pongamia pinnata: an untapped resource for the biofuels: industry of the future. Bioenergy Res 1:2–11
Syros T, Yupsanis T, Economou A (2003) Fractionation and electrophoretic patterns of storage proteins of Ebenus cretica. A preliminary survey as a tool in taxonomy. Biol Plant 46:435–443
Vitale A, Bollini R (1995) Legume storage proteins. In: Kigel J, Galili G (eds) Seed development and germination. Marcel Dekker Inc, New York, pp 73–102
Acknowledgments
VK thanks Council of Industrial Research (CSIR), Government of India for Senior Research Fellowship (SRF). Thanks to the Forest Department officials of Sila Forest, North Guwahati for kind supply of study material. LR acknowledges funding by the Science and Engineering Research Council, Department of Science and Technology (SERC-DST), Government of India for funding the research.
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Kesari, V., Rangan, L. Electrophoretic pattern of proteins isolated from immatured and matured stages of 10 candidate plus trees of versatile oleaginous legume, Pongamia pinnata (L.) Pierre. Agroforest Syst 84, 157–161 (2012). https://doi.org/10.1007/s10457-011-9450-z
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DOI: https://doi.org/10.1007/s10457-011-9450-z