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IGF-1 and BMP-2 Induces Differentiation of Adipose-Derived Mesenchymal Stem Cells into Chondrocytes-Like Cells

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Abstract

Articular cartilage defects are common, causing significant morbidities. Tissue engineering using pluripotent stem cells is a new promising modality for cartilage repair. In the current study, we investigated the chondrogenesis of rabbit adipose-derived stem cells (ADSCs). We isolated rabbit ADSCs and transfected these cells with constructs encoding human insulin growth like factor 1 (IGF-1) and bone morphogenic protein 2 (BMP-2). We examined the growth and morphology of these transfected cells and their production of type II collagen and MMP-3. We found that IGF-1 and BMP-2 drove the chondrogenesis of ADSCs, which showed mature chondrocyte-like cells and formed cartilage nodules. These cells also produced type II collagen with a reduced production of MMP-3. Our findings suggested that human ADSCs could differentiate into chondrocyte-like cells driven by IGF-1 and BMP-2 and held promises as an abundant and ready source of stem cells for cartilage repair and regeneration.

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Authors and Affiliations

  1. Department of Orthopedics, Shengjing Hospital, China Medical University, 36 San Hao Street, Shenyang, Liaoning, 110004, China

    Chunhou An, Yang Cheng, Quan Yuan & Jianjun Li

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  1. Chunhou An
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  2. Yang Cheng
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Correspondence to Chunhou An.

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Associate Editor Eric M. Darling oversaw the review of this article.

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An, C., Cheng, Y., Yuan, Q. et al. IGF-1 and BMP-2 Induces Differentiation of Adipose-Derived Mesenchymal Stem Cells into Chondrocytes-Like Cells. Ann Biomed Eng 38, 1647–1654 (2010). https://doi.org/10.1007/s10439-009-9892-x

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  • DOI: https://doi.org/10.1007/s10439-009-9892-x

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