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Embroidered and Surface Modified Polycaprolactone-Co-Lactide Scaffolds as Bone Substitute: In Vitro Characterization

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Abstract

The aim of this study was to evaluate an embroidered polycaprolactone-co-lactide (trade name PCL) scaffold for the application in bone tissue engineering. The surface of the PCL scaffolds was hydrolyzed with NaOH and coated with collagen I (coll I) and chondroitin sulfate (CS). It was investigated if a change of the surface properties and the application of coll I and CS could promote cell adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSC). The porosity (80%) and pore size (0.2–1 mm) of the scaffold could be controlled by embroidery technique and should be suitable for bone ingrowth. The treatment with NaOH made the polymer surface more hydrophilic (water contact angle dropped to 25%), enhanced the coll I adsorption (up to 15%) and the cell attachment (two times). The coll I coated scaffold improved cell attachment and proliferation (three times). CS, as part of the artificial matrix, could induce the osteogenic differentiation of hMSC without other differentiation additives. The investigated scaffolds could act not just as temporary matrix for cell migration, proliferation, and differentiation in bone tissue engineering but also have a great potential as bioartificial bone substitute.

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Acknowledgments

The authors would like to acknowledge the Bundesminesteriunm für Bildung und Forschung (0314041C) and the company Möckel embroidery and engineering (Auerbach, Germany) for producing the PCL scaffolds.

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Authors and Affiliations

  1. Catgut GmbH, Gewerbepark 18, 08258, Markneukirchen, Germany

    Barbe Rentsch

  2. Max Bergmann Center of Biomaterials, Budapester Straße 27, 01069, Dresden, Germany

    Andre Hofmann & Dieter Scharnweber

  3. Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, 01069, Dresden, Germany

    Annette Breier

  4. Trauma and Reconstructive Surgery, University Hospital Carl Gustav Carus Dresden, Fetscher Straße 74, 01307, Dresden, Germany

    Claudia Rentsch

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  1. Barbe Rentsch
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  2. Andre Hofmann
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  4. Claudia Rentsch
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  5. Dieter Scharnweber
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Correspondence to Barbe Rentsch.

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Rentsch, B., Hofmann, A., Breier, A. et al. Embroidered and Surface Modified Polycaprolactone-Co-Lactide Scaffolds as Bone Substitute: In Vitro Characterization. Ann Biomed Eng 37, 2118–2128 (2009). https://doi.org/10.1007/s10439-009-9731-0

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  • DOI: https://doi.org/10.1007/s10439-009-9731-0

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