Abstract
It was found that Azospirillum brasilense strain Sp7 is able to produce extracellular proteolytic enzymes. The enzymes were active within a broad range of pH values, with two peaks of activity being located in the acid and alkaline pH areas; required calcium ions; and exhibited substrate specificity with respect to azogelatin. Zymography allowed at least four proteolytic enzymes with molecular weights of 32, 45, 52, and 174 kDa to be detected in A. brasilense Sp7 culture liquid. It was shown that the lectin from A. brasilense Sp7 can inhibit proteolytic enzymes.
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Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 4, 2005, pp. 444–448.
Original Russian Text Copyright © 2005 by Chernyshova, Alen’kina, Nikitina, Ignatov.
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Chernyshova, M.P., Alen’kina, S.A., Nikitina, V.E. et al. Extracellular Proteolytic Enzymes of Azospirillum brasilense Strain Sp7 and Regulation of Their Activity by a Homologous Lectin. Appl Biochem Microbiol 41, 390–393 (2005). https://doi.org/10.1007/s10438-005-0066-9
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DOI: https://doi.org/10.1007/s10438-005-0066-9