Summary
Growing three-dimensional tissue within a chamber requires vigorous angiogenesis initiated by, for example, an arteriovenous fistula or a ligated vascular pedicle. Growth may also be enhanced by contact with the external environment. In this study tissue growth in a rat model, vascularized via an arteriovenous loop (AV Loop) or ligated pedicle, was compared in chambers that were either closed or perforated. Chambers were harvested at 4 weeks and tissue volume and histology compared. In perforated chambers, more tissue were generated using the ligated pedicle (0.75 ml±0.04) than the AV Loop (0.59 ml±0.01). Perforated chambers generated larger volumes of tissue than closed chambers because they encouraged tissue ingrowth through the perforations. Both vessel configurations supported tissue growth but, interestingly, the ligated pedicle resulted in significantly more tissue in the perforated chambers.
Zusammenfassung
Die Generierung dreidimensionaler Gewebekomplexe innerhalb einer Wachstumskammer setzt eine intensive Angiogenese voraus, die beispielsweise durch arteriovenöse Shunts oder einen ligierten Gefäßstiel induziert werden kann. Der Wachstumsprozess lässt sich weiter durch Kontakt mit dem umgebendem Gewebe stimulieren. In dieser Studie wurde das angiofibrinöse Gewebewachstum von arteriovenösen Shunts (AV-Shunts) und ligierten Gefäßstielen in perforierten sowie geschlossenen Kammern im Rattenmodell verglichen. Die Kammern wurden nach vier Wochen entfernt, das Volumen des Gewebes bestimmt und histomorphometrisch analysiert. In perforierten Kammern wurde mit ligierten Gefäßstielen (0,75 ml±0,04) mehr Gewebe als mit AV-Shunts (0,59 ml±0,01) generiert. Die perforierten Kammern produzierten größere Volumina als der geschlossene Typ, da sie eine zusätzliche Angiogenese durch die Perforationen erlaubten. Beide Gefäßstielkonfigurationen förderten das Wachstum von Gewebe, jedoch resultierte interessanterweise aus ligierten Gefäßstielen signifikant mehr Gewebe in den perforierten Modellen.
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Dolderer, J., Kehrer, A., Schiller, S. et al. De-novo Generierung von vaskularisiertem Gewebe mittels unterschiedlicher Gefässstielkonfigurationen in perforierten und geschlossenen Wachstumskammern. Wien Med Wochenschr 160, 139–146 (2010). https://doi.org/10.1007/s10354-009-0734-0
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DOI: https://doi.org/10.1007/s10354-009-0734-0
Keywords
- Tissue engineering
- Angiogenesis
- Autologous soft tissue replacement
- Reconstructive surgery
- Growth chambers