Abstract
Aujeszky’s disease is an economically important disease in domestic pigs caused by the alphaherpesvirus pseudorabies virus (PRV). However, also wild boars are a natural reservoir for the virus, and this can lead to infection of wildlife carnivore species. Three wolves held in the wildlife park of Han-sur-Lesse in the province of Namur in Belgium were suspected to be infected with PRV based on the nervous symptoms they showed after being fed with wild boar offal. The diagnosis was confirmed for a female wolf by a positive real-time PCR detecting PRV. The virus was isolated from the brain tissue of the wolf and characterized by restriction fragment length polymorphism analysis and phylogenetic analysis. The obtained BamHI restriction fragment pattern of the wolf isolate was similar to that of the reference strain Kaplan, thereby characterizing it as a type Ip isolate. Type I PRV strains, and particularly subtype Ip, are predominant in European wild boar. Phylogenetic analysis based on the sequence of a fragment of glycoprotein C showed that the Belgian isolate belonged to cluster B and that the sequence was identical to that of wild boar isolates from southwestern Germany, eastern France, and Spain. This study is the first report of Aujeszky’s disease in wolves and shows that they are susceptible to PRV by eating infected wild boar offal leading to fatal neurological disease. This illustrates the possible implications of PRV-infected wild boar for the conservation of wolves and other carnivore species.
References
Bitsch V, Knox B, Munch B (1969) Cases of pseudorabies in free-living red foxes (Vulpes vulpes) and in captive blue foxes (Alopex lagopus) in Denmark. Acta Vet Scand 10:195–196
Capua I, Casaccia C, Calzetta G, Caporale V (1997) Characterisation of Aujeszky’s disease viruses isolated from domestic animals and from a wild boar (Sus scrofa) in Italy between 1972 and 1995. Vet Microbiol 57:143–149
Czaplicki G, Dufey J, Saegerman C (2006) Le Sanglier Wallon est-il un reservoir potential du virus de la maladie d’Aujeszky pour les elevages porcins? Epidémiol Santé Anim 49:89–101
Glass CM, McLean RG, Katz JB, Maehr DS, Cropp CB, Kirk LJ, McKeirnan AJ, Evermann JF (1994) Isolation of pseudorabies (Aujeszky’s disease) virus from a Florida panther. J Wildl Dis 30:180–184
Goldberg TL, Weigel RM, Hahn EC, Scherba G (2001) Comparative utility of restriction fragment length polymorphism analysis and gene sequencing to the molecular epidemiological investigation of a viral outbreak. Epidemiol Infect 126:415–424
Gortázar C, Vincente J, Fierro Y, León L, Cubero MJ, González M (2002) Natural Aujeszky’s disease in a Spanish wild boar population. Ann N Y Acad Sci 969:210–212
Haddane B, Essalhi A (1998) An outbreak of Aujeszky’s disease in the hunting dog (Lycaon pictus). In: European Association of Zoo- and Wildlife disease Veterinarians (EAZWV) Second scientific meeting, May 21–24, 1998, Chester, United Kingdom
Hahn EC, Fadl-Alla B, Lichtensteiger CA (2010) Variation of Aujeszky’s disease viruses in wild swine in USA. Vet Microbiol 143:45–51
Herrmann S, Heppner B, Ludwig H (1984) Pseudorabies viruses from clinical outbreaks and latent infections grouped into four major genome types. Curr Top Vet Med Anim Sci 27:387–401
Kaden V, Lange E, Fischer U, Strebelow G (2000) Oral immunization of wild boar against classical swine fever: evaluation of the first field study in Germany. Vet Microbiol 73:239–252
Maresch C, Lange E, Teifke JP, Fuchs W, Klupp B, Müller T, Mettenleiter TC, Vahlenkamp TW (2012) Oral immunization of wild boar and domestic pigs with attenuated live vaccine protects against Pseudorabies virus infection. Vet Microbiol 161:20–25
Müller T, Klupp BG, Freuling C, Hoffmann B, Mojcicz M, Capua I, Palfi V, Toma B, Lutz W, Ruiz-Fon F, Gortárzar C, Hlinak A, Schaarschmidt U, Zimmer K, Conraths FJ, Hahn EC, Mettenleiter TC (2010) Characterization of pseudorabies virus of wild boar originating from Europe. Epidemiol Infect 138:1590–1600
Müller T, Hahn EC, Tottewitz F, Kramer M, Klupp BG, Mettenleiter TC, Freuling C (2011) Pseudorabies virus in wild swine: a global perspective. Arch Virol 156:1691–1705
Nores C, Llaneza L, Álvarez Á (2008) Wild boar Sus scrofa mortality by hunting and wolf Canis lupus predation: an example in northern Spain. Wildl Biol 14:44–51
Pomeranz LE, Reynolds AE, Hengartner CJ (2005) Molecular biology of pseudorabies virus: impact on neurovirology and veterinary medicine. Microbiol Mol Biol Rev 69:462–500
Raymond JT, Gillespie RG, Woodruff M, Janovitz EB (1997) Pseudorabies virus in captive coyotes. J Wildl Dis 33:916–918
Schultze AE, Maes RK, Taylor DC (1986) Pseudorabies and volvulus in a black bear. J Am Vet Med Assoc 189:1165–1166
Schulze C, Hlinak A, Wohlsein P, Kutze P, Müller T (2010) Spontaneous Aujeszky’s disease (pseudorabies) in European wild boars (Sus scrofa) in the federal state of Brandenburg, Germany. Berl Munch Tierarztl Wochenschr 123:359–364
Steinrigl A, Revilla-Fernández S, Kolodziejek J, Wodak E, Bagó Z, Nowotny N, Schmoll F, Köfer J (2012) Detection and molecular characterization of Suid herpesvirus type 1 in Austrian wild boar and hunting dogs. Vet Microbiol 157:276–284
Zanin E, Capua I, Casaccia C, Zuin A, Moresco A (1997) Isolation and characterization of Aujeszky’s disease virus in captive brown bears from Italy. J Wildl Biol 33:632–634
Acknowledgments
We would like to thank Prof. H. Favoreel and Prof. H. Nauwynck from Ghent University for the kind gift of the PRV reference strains Bartha and Kaplan. This study was funded by the Belgian Federal Public Service of Health, Food Chain Safety and Environment (RF11/6249).
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Communicated by C. Gortazar
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Verpoest, S., Cay, A.B., Bertrand, O. et al. Isolation and characterization of pseudorabies virus from a wolf (Canis lupus) from Belgium. Eur J Wildl Res 60, 149–153 (2014). https://doi.org/10.1007/s10344-013-0774-z
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DOI: https://doi.org/10.1007/s10344-013-0774-z