Abstract
The development of European plum (Prunus domestica L.) cultivars is a crucial part of the 70-year-long tradition of breeding work on temperate zone fruit species at the Fruit Research Institute, Čačak, Republic of Serbia. Since its establishment to date, 18 plum cultivars of worldwide importance have been named and released. During the evaluation of newly bred plum genotypes, particular attention has been paid to the study of their self-(in)compatibility status, which is very important both for growers and breeders. Therefore, the objective of this study was to determine the self-(in)compatibility status of the recently released plum cultivars ‘Divna’, ‘Lana’ and ‘Petra’. The 2‑year (2018/19) study included: assessment of in vitro pollen germination on agarose-sucrose medium, observation of pollen tube growth in vivo in pistils under self- and open-pollination modes using fluorescence microscopy and determination of fruit set. In addition, the effect of temperature on the progamic phase was evaluated. In vitro pollen germination had high values, indicating the good in vivo pollen behaviour. High values of the parameters of pollen tube growth in vivo and fruit set in both self- and open-pollination variants were obtained in ‘Divna’ and ‘Petra’. On the other hand, pollen tube growth inhibition was observed in the upper part of the styles after self-pollination of ‘Lana’. Consequently, in the base of the style and in the ovary no tubes were noticed and no fruit set was recorded. Based on the overall results, ‘Lana’ can be characterized as self-incompatible, whilst ‘Divna’ and ‘Petra’ are self-compatible plum cultivars. The influence of air temperatures on the reproductive process and fertilization success was specific to each genotype.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdallah D, Baraket G, Perez V, Ben Mustapha S, Salhi-Hannachi A, Hormaza JI (2019) Analysis of self-incompatibility and genetic diversity in diploid and hexaploid plum genotypes. Front Plant Sci 10:896. https://doi.org/10.3389/fpls.2019.00896
Ćalić D, Devrnja N, Kostić I, Kostić M (2013) Pollen morphology, viability, and germination of prunus domestica cv. Požegača. Sci Hortic 155:118–122. https://doi.org/10.1016/j.scienta.2013.03.017
Cerovic R, Mićić N (1996) Oprašivanje i oplodnja jabučastih i koštičavih voćaka. Jugosl Voćarstvo 30:73–98
Cerović R, Ružić Đ (1992) Pollen tube growth in sour cherry (prunus cerasus l.) at different temperatures. J Hortic Sci 67:333–340
Cerović R, Ružić Đ, Mićić N (2000) Viability of plum ovules at different temperatures. Ann Appl Biol 137:53–59. https://doi.org/10.1111/j.1744-7348.2000.tb00056.x
Đorđević M, Cerović R, Radičević S, Nikolić D, Glišić IS, Milošević N, Marić S, Lukić M (2019) Pollen tube growth and embryo sac development in ‘Pozna Plava’ plum cultivar related to fruit set. Erwerbs-Obstbau 61:313–322. https://doi.org/10.1007/s10341-019-00431-7
Fotirić-Akšić M, Cerović R, Harald-Hjeltnes S, Meland M (2022) The effective pollination period of European plum (prunus domestica l.) cultivars in Western Norway. Horticulturae 8:55. https://doi.org/10.3390/horticulturae8010055
Glišić IS, Milatović D, Cerović R, Radičević S, Đorđević M, Milošević N (2017) Examination of self-compatibility in promising plum (prunus domestica l.) genotypes developed at the fruit research institute, Čačak. Sci Hortic 224:156–162. https://doi.org/10.1016/j.scienta.2017.06.006
Guerra ME, Rodrigo J (2015) Japanese plum pollination: a review. Sci Hortic 197:674–686. https://doi.org/10.1016/j.scienta.2015.10.032
Halász J, Hegedüs A (2006) A critical evaluation of methods used for S‑genotyping: from trees to DNA level. Int J Hort Sci 12(2):19–29
Halász J, Hegedüs A, Szabó Z, Nyéki J, Pedryc A (2007) DNA-based S‑genotyping of Japanese plum and pluot cultivars to clarify incompatibility relationships. Hortic Sci 42(1):46–50. https://doi.org/10.21273/HORTSCI.42.1.46
Halász J, Kurilla A, Hegedûs A (2014) Preliminary characterization of the self-incompatibility genotypes of European plum (prunus domestica l.) cultivars. Int J Hortic Sci 20:23–26
Halász J, Makovics-Zsohàr N, Szöke F, Ercisli S, Hegedüs A (2017) Simple sequence repeat and S‑locus genotyping to explore genetic variability in polyploid prunus spinosa and p. institia. Biochem Genet 55:22–33. https://doi.org/10.1007/s10528-016-9768-3
Hartmann W, Neumüller M (2009) Plum breeding. In: Jain SM, Priyadarshan PM (eds) Breeding plantation tree crops: temperate species. Springer, New York, pp 161–232
Hedhly A, Hormaza JI, Herrero M (2003) The effect of temperature on stigmatic receptivity in sweet cherry (prunus avium l.). Plant Cell Environ 26:1673–1680. https://doi.org/10.1046/j.1365-3040.2003.01085.x
Hedhly A, Hormaza JI, Herrero M (2004) Effect of temperature on pollen tube kinetics and dynamics in sweet cherry, prunus avium (rosaceae). Am J Bot 91:558–564. https://doi.org/10.3732/ajb.91.4.558
Hedhly A, Hormaza JI, Herrero M (2005) Influence of genotype-temperature interaction on pollen performance. J Evol Biol 18:1494–1502. https://doi.org/10.1111/j.1420-9101.2005.00939.x
Hedhly A, Hormaza JI, Herrero M (2009) Global warming and sexual plant reproduction. Trends Plant Sci 14:30–36. https://doi.org/10.1016/j.tplants.2008.11.001
Herrero M (1992) Mechanisms in the pistil that regulate gametophyte population in peach (prunus persica). In: Ottaviano E, Mulcahy DL, Sari-Gorla M, Mulcahy GB (eds) Angiosperm pollen and ovules. Springer, Berlin, Heidelberg, pp 377–381
Hormaza JI, Herrero M (1996) Dynamic of pollen tube growth under different competition regimes. Sex Plant Reprod 9:153–160. https://doi.org/10.1007/BF02221395
Ivanov MK, Dymshits GM (2007) Cytoplasmic male sterility and restoration of pollen fertility in higher plants. Russ J Genet 43(4):354–368. https://doi.org/10.1134/S1022795407040023
Keulemans J (1984) The effect of temperature on pollen tube growth and fruit set on plum trees. Acta Hortic 149:95–101. https://doi.org/10.17660/ActaHortic.1984.149.11
Kho YO, Baër J (1971) Fluorescence microscopy in botanical research. Zeiss Inf 76:54–57
Kota-Dombrovska I, Lãcis G (2013) Evaluation of self-incompatibility locus diversity of domestic plum (prunus domestica l.) using DNA-based S‑genotyping. Proc Latv Acad Sci B Nat Exact Appl Sci 67:109–115. https://doi.org/10.2478/prolas-2013-0017
Kumar AS, Geethanjali K (2018) Molecular mechanism of the self-incompatibility in flowering plants. Bull Environ Pharmacol Life Sci 7:164–169
Makovics-Zsohár N, Halász J (2016) Self-incompatibility system in polyploid fruit tree species—A review. Int J Plant Reprod Biol 8(1):24–33. https://doi.org/10.14787/ijprb.2016 8.1.24–33
Meier U (2018) Growth stages of mono- and dicotyledonous plants, 2nd edn. BBCH monograph. Julius Kühn-Institut, Quedlinburg, pp 1–204
Meland M, Maas FM (2017) Regulation of fruiting in plum production. Proceedings of the 6th Conference “Innovation in Fruit Growing”. Faculty of Agriculture, Belgrade, pp 51–67
Neumüller M (2011) Fundamental and applied aspects of plum (prunus domestica l.) breeding. Fruit Veg Cereal Sci Biotechnol 5:139–154
Nikolić D, Milatović D (2010) Examining self-compatibility in plum (prunus domestica l.) by fluorescence microscopy. Genetika 42:387–396. https://doi.org/10.2298/GENSR1002387N
Nikolić D, Rakonjac V, Fotirić-Akšić M (2012) The effect of pollinizer on the fruit set of plum cultivar Čačanska Najbolja. J Agric Sci 57:9–18. https://doi.org/10.2298/JAS1201009N
Nyéki J, Szabó Z (1995) Cross-incompatibility in stone fruits. Hort Sci 27:23–31
Paunović SA, Glišić IS, Karaklajić-Stajić Ž, Milošević N, Jevremović D (2021) Performance of ‘Lana’, a new plum cultivar developed at the fruit research institute, Čačak. Acta Hortic 1322:25–32. https://doi.org/10.17660/ActaHortic.2021.1322.5
Preil W (1970) Observing of pollen tube in pistil and ovarian tissue by means of fluorescence microscopy. Zeiss Inf 75:24–25
Radičević S, Cerović R, Nikolić D, Đorđević M (2016) The effect of genotype and temperature on pollen tube growth and fertilization in sweet cherry (prunus avium l.). Euphytica 209:121–136. https://doi.org/10.1007/s10681-016-1645-y
Scott RJ, Spielman M, Dickinsonb HG (2004) Stamen structure and function. Plant Cell 16:46–60. https://doi.org/10.1105/tpc.017012
Stone SL, Goring DR (2001) The molecular biology of self-incompatibility systems in flowering plants. Plant Cell Tissue Organ Cult 67:93–114. https://doi.org/10.1023/A:1011980210048
Sutherland BG, Robbins TP, Tobutt KR (2004) Primers amplify a range of prunus S‑alleles. Plant Breed 123:582–584. https://doi.org/10.1111/j.1439-0523.2004.01016.x
Sutherland BG, Tobutt KR, Robbins PТ (2008) Trans-specific S‑RNase and SFB alleles in prunus self-incompatibility haplotypes. Mol Genet Genomics 279:95–106. https://doi.org/10.1007/s00438-007-0300-7
Sutherland BG, Cerović R, Robbins TP, Tobutt KR (2009) The myrobalan (prunus cerasifera l.): a useful diploid model for studying the molecular genetics of self-incompatibility in plums. Euphytica 166:385–398. https://doi.org/10.1007/s10681-008-9821-3
Szabó Z (2003) Plum (prunus domestica l.). In: Kozma P, Nyéki M, Szabó Z (eds) Floral biology, pollination and fertilisation in temperate zone fruit species and grape. Akadémiai Kiadó, Budapest, pp 383–410
Viti R, Monteleone P, Guerriero R (1997) Incompatibility in apricot (prunus armeniaca l.): methodological considerations. J Hortic Sci 72:961–970. https://doi.org/10.1080/14620316.1997.11515588
Wertheim SJ (1996) Methods for cross pollination and flowering assessment and their interpretation. Acta Hortic 423:237–241. https://doi.org/10.17660/ActaHortic.1996.423.30
Zhebentyayeva T, Shankar V, Scorza R, Callahan A, Ravelonandro M, Castro S, DeJong T, Saski CA, Dardick C (2019) Genetic characterization of worldwide prunus domestica (plum) germplasm using sequence-based genotyping. Hortic Res 6(12):1–13. https://doi.org/10.1038/s41438-018-0090-6
Acknowledgements
This work was supported by the Ministry of Science, Technological Development and Innovation of the Republic of Serbia (Contract number: 451-03-47/2023-01/200215).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
I. Glišić, M. Đorđević, N. Milošević, S. Radičević, S. Marić and R. Cerović declare that they have no competing interests.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Glišić, I., Đorđević, M., Milošević, N. et al. Reproductive Behaviour of New European Plum Cultivars ‘Lana’, ‘Divna’ and ‘Petra’. Erwerbs-Obstbau 65, 2379–2389 (2023). https://doi.org/10.1007/s10341-023-00972-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10341-023-00972-y