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Graphene Adsorbent-Based Solid-Phase Extraction for Aflatoxins Clean-Up in Food Samples

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Abstract

In this research, graphene nanoparticles were used as an adsorbent for the extraction and pre-concentration of the aflatoxins (AFs) in rice and wheat samples. The graphene was synthesized according to the Hummer’s method, packed in empty SPE cartridges and different parameters affecting the efficiency of the process for the extraction of AFs were thoroughly investigated. The extracts were subsequently analyzed using high-performance liquid chromatography and fluorescence detection (HPLC–FLD). Under the optimized conditions, limits of detection of 0.63, 0.47, 0.62 and 0.83 ng g−1 and limits of quantification of 1.92, 2.65, 1.88 and 2.83 ng g−1 were obtained for AFs B1, B2, G1 and G2, respectively. The accuracy was estimated by determination of the relative recoveries (RR%) and acceptable values obtained. The RR% values of 75.88–113.30 and 70.61–110.75 were obtained in cereal samples and at the spiked level of 2 and 5 ng g−1, respectively. Within-laboratory relative standard deviations (%RSDs) for repeatability (n = 6) were in the range of 2.14–3.17. The proposed new method can be applied for the separation and pre-concentration of the mentioned AFs in food samples with satisfactory results.

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Acknowledgements

This research is supported by Iranian National Science Foundation (INSF) for supporting this work financially under the contract number 94/SS/45515.

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Authors and Affiliations

  1. Department of Food Quality Control and Safety, Research Institute of Food Science and Technology (RIFST), P.O. Box 91735-147, Mashhad, Iran

    Javad Feizy

  2. Department of Food Chemistry, Research Institute of Food Science and Technology, Mashhad, Iran

    Moslem Jahani & Adel Beigbabaei

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  1. Javad Feizy
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  2. Moslem Jahani
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Correspondence to Javad Feizy.

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Real samples were just food such as wheat and rice samples. Human samples have not been used in this study.

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Feizy, J., Jahani, M. & Beigbabaei, A. Graphene Adsorbent-Based Solid-Phase Extraction for Aflatoxins Clean-Up in Food Samples. Chromatographia 82, 917–926 (2019). https://doi.org/10.1007/s10337-019-03725-w

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