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A Multiresidue Method for Simultaneous Determination of 116 Pesticides in Notoginseng Radix et Rhizoma Using Modified QuEChERS Coupled with Gas Chromatography Tandem Mass Spectrometry and Census 180 Batches of Sample from Yunnan Province

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Abstract

A method was established for the simultaneous determination of 116 pesticide residues in Notoginseng Radix et Rhizome with a combination of the modified QuEChERS method and GC–MS/MS. The sample was extracted with acetonitrile, cleaned up by primary–secondary amine and octadecyl-modified silica (C18) sorbents and determined by GC–MS/MS in multireaction monitoring mode. Matrix-matched calibration coupled with internal standard method was applied to compensate for the matrix effect and to quantify the pesticides. The results of all the 116 pesticides showed good linearity in the respective linear range with correlation coefficients (r2) > 0.99. The method limits of quantification were between 0.01 and 0.05 mg kg−1. The recoveries were between 64.3 and 119.4%, with RSD values typically lower than 18.3% at three spiked levels of 0.05, 0.10 and 0.20 mg kg−1. The validated methodology is easy, fast, highly accurate, reliable and sensitive for monitoring and quantification of the 116 pesticide residues in Notoginseng Radix et Rhizoma. In 180 batches of real samples, 11 pesticides were detected and among these quintozene and cyfluthrin were in excess of the standard of European Union maximum residue level for herbs.

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Acknowledgements

This work was supported by the National Science and Technology Major Projects for “Major New Drugs Innovation and Development” (no. 2013ZX09402202).

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Authors and Affiliations

  1. School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, Liaoning, People’s Republic of China

    Xiyu Duan, Zhiguo Yu & Yunli Zhao

  2. State Key Laboratory of Core Technology in Innovative Chinese Medicine, Pharmaceutical Analysis Institute, Tasly Academy, Tianjin, 300410, People’s Republic of China

    Ling Tong & Dongxiang Li

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  1. Xiyu Duan
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  2. Ling Tong
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  3. Dongxiang Li
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  4. Zhiguo Yu
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Correspondence to Yunli Zhao.

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Funding

This study was funded by the Department of Science and Technology for Social Development of The People’s Republic of China (Grant number 2013ZX09402202).

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All the authors report no conflicts of interest with this study.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Duan, X., Tong, L., Li, D. et al. A Multiresidue Method for Simultaneous Determination of 116 Pesticides in Notoginseng Radix et Rhizoma Using Modified QuEChERS Coupled with Gas Chromatography Tandem Mass Spectrometry and Census 180 Batches of Sample from Yunnan Province. Chromatographia 81, 545–556 (2018). https://doi.org/10.1007/s10337-017-3460-6

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  • DOI: https://doi.org/10.1007/s10337-017-3460-6

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