Abstract
An improved solvent bar microextraction (SBME) combined with HPLC was applied to rapidly determine oleanolic acid (OA) and ursolic acid (UA) in Chinese medicinal herbs (CMHs), and to investigate drug–protein binding. Variables influencing SBME were investigated and optimized. Under optimal conditions, the linear ranges of 3.6–1,820 ng mL−1 for OA and 4.2–2,080 ng mL−1 for UA were obtained with square correlation coefficients of 0.9996 and 0.9997, respectively. The detection limits were 1.3 ng mL−1 for OA and 1.5 ng mL−1 for UA. The relative standard deviations were lower than 9.4 %. The protein-binding rates, the numbers of binding sites, and the binding constants of OA and UA were also obtained via the method. It has been demonstrated that SBME can not only be a simple, rapid and efficient sample preparation method for determination of active components from CMHs but also be a potential research protocol for protein-binding interactions.
This is a preview of subscription content, log in via an institution to check access.
References
Mandal V, Mandal SC (2010) Biochem Eng J 50:63–70. doi:10.1097/PAP.0b013e318032508d
Kazmi I, Rahman M, Afzal M, Gupta AG, Saleem S, Afzal O, Shaharyar MA, Nautiyal U, Ahmed S, Anwar F (2012) Fitoterapia 83:142–146. doi:10.1016/j.fitote.2011.10.004
Xu XH, Su Q, Zang ZH (2012) J Pharm Anal 2:238–240. doi:10.1016/j.jpha.2012.01.006
Wang HH, Wang ZZ, Guo WB (2008) J Ind Crop Prod 28:328–332. doi:10.1016/jndcrop.2008.03.004
Li GL, Zhang XL, You JM, Song CH, Sun ZW, Xia L, Suo YR (2011) Anal Chim Acta 688:208–218. doi:10.1016/j.aca.2011.01.010
Wójciak-Kosior M (2007) J Pharm Biomed Anal 45:337–340. doi:10.1016/j.jpba.2007.05.011
Guo X, Cheng ZN, Cao W, Liang HX, Huang ZZ, Li HD (2008) Chin J Hosp Pharm 28:599–602
Zhang H, Zhang HF, Chang HC, Han X, Bi KS, Chen XH (2011) Acta Pharmaceutica Sinica 46:243–246
Zhang LY, Guo M, Chen JY, Li MH, Wu MY (2010) Chem Ind Forest Prod 30:41–48
Pedersen-Bjergaard S, Rasmussen KE (1999) Anal Chem 71:2650–2656. doi:10.1021/ac990055n
Wang J, Hu S, Bai XH (2010) Chromatographia 72:453–458. doi:10.1365/s10337-010-1686-70009-5893/10/09
Ma WY, Chen X, Hu S, Bai XH. Anal Lett. Doi:10.1080/00032719.2013.836658
Hu S, Zhang YJ, Bai XH (2011) Chromatographia 74:503–506. doi:10.1007/s10337-011-2103-6
Zhang X, Chen X, Bai XH (2011) Chin J. Anal Chem 39:857–862. doi:10.3724/SP.J.1096.2011.00857
Zhang M, Li Q, Ji W, Jiang S, Ma C, Wang C, Ye J, Cui Y, Liu WT, Bi KS, Chen XH (2011) Chromatographia 73:897–903. doi:10.1007/s10337-011-1982-x
Acknowledgments
We thank the Nature Science Foundation of Shanxi Province (No. 2012011007-4), the Innovation Foundation of Shanxi Medical University (No. C01201011), and the Program for the Top Science and Technology Innovation Teams of Higher Learning Institutions of Shanxi Province, China (2011) for the financial support.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Hao, Y., Chen, X., Hu, S. et al. Solvent Bar Microextraction with HPLC for Determination and Protein-Binding Characteristics of Oleanolic Acid and Ursolic Acid. Chromatographia 77, 359–363 (2014). https://doi.org/10.1007/s10337-013-2599-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10337-013-2599-z