Abstract
Pregnancy alters pharmacokinetic profile of many drugs, because of altering body volume and metabolism rate. Therefore, dosage rates and concentrations of drugs must be controlled during pregnancy. Here, we identified the pharmacokinetic profile of pre-operatively given cefepime in caesarean section and gynecological operations using a simple, rapid, cost-effective and valid liquid chromatographic method. The chromatographic separation was performed using 40 mM, pH 3.2 phosphate buffer containing 6 % methanol as mobile phase at 0.30 mL min−1 flow rate. Gradient elution with methanol was applied to get shorter analysis time without any interference from plasma endogens. During analyses, temperature of column, autosampler and detector were set as 30, 10 and 40 °C, respectively. The detection wavelength was 260 nm and ceftizoxime was used as internal standard. At the optimum conditions, the cefepime analysis from plasma samples was completed in 7 min. Cefepime was extracted from plasma samples using perchloric acid with a very high recovery rate (99.3 %). The method was fully validated according to the Food and Drug Administration guidelines for bioanalytical method validation, and found to be selective, linear, repeatable, reproducible and robust. After validation studies, the method was applied to five caesarean-sectioned and four non-pregnant sectioned women treated with pre-operative, prophylactic single intravenous dose of cefepime (1 g Maxipime®) in order to determine pharmacokinetic profile of cefepime. Peak serum concentrations of cefepime in caesarean-sectioned women at the arterial port after infusion was 70.11 ± 10.74 μg mL−1. The mean elimination half-life, volume of distribution and calculated area under the concentration–time curve (AUC)0–∞ were 1.10 ± 0.23 h, 14.22 ± 2.29 L and 101.55 ± 10.99 μg h mL−1 for caesarean-sectioned women; and 1.14 ± 0.21 h, 14.76 ± 2.92 L and 104.71 ± 36.34 μg h mL−1 for non-pregnant sectioned women, respectively. The area under curve, elimination half-life, maximum plasma concentration and the mean distribution volume of cefepime were not changed in case of pregnancy.
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References
Barradell LB, Bryson HM (1994) Drugs 47:471–505
Chapman TM, Perry CM (2003) Am J Respir Med 2:75–107
Rybak M (1996) Am J Med 100:39S–44S
Dawes M, Chowienczyk PJ (2001) Best Pract Res Clin Obstet Gynaecol 15:819–826
Nemutlu E, Kir S, Eroglu H, Katlan D, Ozek A, Oyuncu O, Beksac MS (2010) Comb Chem High T Scr 13:502–509
Morgan D, Smallwood R (1990) Clin Pharmacokinet 18:61
Popovi J, Gruji Z, Sabo A (2007) J Clin Pharm Ther 32:595–602
Philipson A (1979) Clin Pharmacokinet 4:297–309
Valassis IN, Parissi-Poulou M, Macheras P (1999) J Chromatogr B 721:249–255
Calahorra B, Campanero MA, Sadaba B, Azanza JR (1999) Biomed Chromatogr 13:272–275
Breilh D, Lavallee C, Fratta A, Ducint D, Cony-Makhoul P, Saux MC (1999) J Chromatogr B 734:121–127
Cherti N, Kinowski JM, Lefrant JY, Bressolle F (2001) J Chromatogr B 754:377–386
Breilh D, Saux MC, Delaisement C, Fratta A, Ducint D, Velly JF, Couraud L (2001) Pulm Pharmacol Ther 14:69–74
Ikawa K, Morikawa N, Hayato S, Ikeda K, Ohge H, Sueda T (2007) Int J Antimicrob Ag 30:270–273
Palacios FJJ, Mochon MC, Sanchez JCJ, Lopez MAB, Perez AG (2005) Chromatographia 62:355–361
Lopez KJV, Bertoluci DF, Vicente KM, Dell’Aquilla AM, Santos SRCJ (2007) J Chromatogr B 860:241–245
Denooz R, Charlier C (2008) J Chromatogr B 864:161–167
Farthing C, Farthing D, Brophy DF, Larus T, Maynor L, Fakhry I, Gehr TWB (2008) Chromatographia 67:365–368
Ozkan Y, Kucukguzel I, Ozkan SA, Aboul-Enein HY (2001) Biomed Chromatogr 15:263–266
Nemutlu E, Kir S, Katlan D, Beksac MS (2009) Talanta 80:117–126
Verdier MC, Tribut O, Tattevin P, Le Tulzo Y, Michelet C, Bentue-Ferrer D (2011) Antimicrob Agents Ch 55:4873–4879
Ohmori T, Suzuki A, Niwa T, Ushikoshi H, Shirai K, Yoshida S, Ogura S, Itoh Y (2011) J Chromatogr B 879:1038–1042
Causon R (1997) J Chromatogr B 689:175–180
Little BB (1999) Obstet Gynecol 93:858–868
Satoskar RS, Bhandarkar SD, Rege NN (2007) Pharmacology and pharmacotherapeutics. Popular prakashan, Mumbai, pp 1091–1101
Azancot-Benisty A, Jacqz-Aigrain E, Guirgis NM, Decrepy A, Oury JF, Blot P (1992) J Pediatr 121:608–613
Gerdin E, Salmonson T, Lindberg B, Rane A (1990) J Perinat Med 18:479–487
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Ayşegül Doğan and Emirhan Nemutlu contributed equally to this work.
Published in the topical collection New Developments for the Sensitive and Validated Drug Analysis Using Chromatographic Methods with guest editor Sibel A. Ozkan.
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Doğan, A., Nemutlu, E., Özek, M.A. et al. The Pharmacokinetic Profiles of Pre-Operative Prophylactic Cefepime Application in Pregnant and Non-Pregnant Women Undergoing Surgical Interventions Using a Fully Validated Liquid Chromatographic Method. Chromatographia 76, 1513–1519 (2013). https://doi.org/10.1007/s10337-013-2415-9
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DOI: https://doi.org/10.1007/s10337-013-2415-9