Skip to main content

Advertisement

SpringerLink
Log in

Tissue oxygen tension measurements in the Shionogi model of prostate cancer using 19F MRS and MRI

  • Research Article
  • Published:
Magnetic Resonance Materials in Physics, Biology and Medicine Aims and scope Submit manuscript

Abstract

Objectives: To investigate changes in tumour tissue oxygenation throughout the tumour growth–regression–relapse cycle in an androgen-dependent animal tumour model. Materials and methods: 19F T1 relaxometry of Perfluoro-15-Crown-5-Ether was used to measure in vivo partial oxygen pressure (pO2) of Shionogi tumours on a 2.35-T MR scanner. Perfluoro-15-Crown-5-Ether was administered as an emulsion injected intravenously or as a neat compound injected directly into the tumour. Non-localized, tumour 19F T1 measurements, made at multiple time points throughout the tumour cycle, were translated into pO2 levels. Results: No correlation between tumour size and pO2 values was found. Values of pO2 for growing tumours (50 ± 30 torr) were significantly lower than for regressing and relapsing tumours after 9 days post-castration (70 ± 10 torr, p<0.05). Maximum pO2 values (90 ± 30 torr) were reached between fifth and eighth day post-castration, when tumour pO2 was significantly higher than both pre-castration (p<0.001) and after 9 days post-castration (p<0.05). Conclusion: We demonstrate that longitudinal pO2 measurements in vivo are feasible. Values of pO2 for growing androgen-dependent tumours were significantly lower than for regressing and relapsing androgen-independent tumours. These results have potential clinical importance in optimizing the timing of chemotherapy and/or radiotherapy of hormone dependent tumours.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gillies RJ, Raghunand N, Karczmar GS, Bhujwalla ZM (2002) MRI of the tumor microenvironment. J Magn Reson Imaging 16:430–450

    PubMed  Google Scholar 

  2. Teicher BA (1995) Physiologic mechanisms of therapeutic resistance. Blood flow and hypoxia. Hematol Oncol Clin North Am 9:475–506

    CAS  PubMed  Google Scholar 

  3. De Jaeger K, Kavanagh MC, Hill RP (2001) Relationship of hypoxia to metastatic ability in rodent tumours. Br J Cancer 84:1280–1285

    Google Scholar 

  4. Rofstad EK, Danielsen T (1999) Hypoxia-induced metastasis of human melanoma cells: involvement of vascular endothelial growth factor-mediated angiogenesis. Br J Cancer 80:1697–1707

    PubMed  CAS  Google Scholar 

  5. Miayake H, Tolcher A, Gleave ME (2000) Chemosensitization and delayed androgen-independent recurrence of prostate cancer with the use of antisense Bcl-2 oligodeoxynucleotides. J Natl Cancer Inst 92:34–41

    PubMed  CAS  Google Scholar 

  6. Minesita T, Yamaguchi K (1964) An androgen-dependent tumour derived from a hormone-independent spontaneous tumour of a female mouse. Steroids 4:815–829

    Google Scholar 

  7. Rennie PS, Bruchovsky N, Buttyan R, Benson M, Cheng H (1988) Gene expression during the early phases of regression of the androgen-dependent Shionogi mouse mammary carcinoma. Cancer Res 48:6309–6312

    PubMed  CAS  Google Scholar 

  8. Parhami P, Fung BM (1983) Fluorine-19 relaxation study of perfluoro chemicals as oxygen carriers. J Phys Chem 87:1928–1931

    CAS  Google Scholar 

  9. Mason RP, Antich PP (1994) Tumor oxygen tension: measurement using Oxygent as a 19F NMR probe at 4.7 T. Artif Cells Blood Substit Immobil Biotechnol 22:1361–1367

    PubMed  CAS  Google Scholar 

  10. Mason RP, Shukla H, Antich PP (1992) Oxygent: a novel probe of tissue oxygen tension. Biomater Artif Cells Immobilization Biotechnol 20:929–932

    PubMed  CAS  Google Scholar 

  11. Le D, Mason RP, Hunjan S, Constantinescu A, Barker BR, Antich PP (1997) Regional tumor oxygen dynamics: 19F PBSR EPI of hexafluorobenzene. Magn Reson Imaging 15:971–981

    PubMed  CAS  Google Scholar 

  12. Zhao D, Constantinescu A, Jiang L, Hahn EW, Mason RP (2001) Prognostic radiology: quantitative assessment of tumor oxygen dynamics by MRI. Am J Clin Oncol 24:462–466

    PubMed  CAS  Google Scholar 

  13. Hunjan S, Zhao D, Constantinescu A, Hahn EW, Antich PP, Mason RP (2001) Tumor oximetry: demonstration of an enhanced dynamic mapping procedure using fluorine-19 echo planar magnetic resonance imaging in the Dunning prostate R3327-AT1 rat tumor. Int J Radiat Oncol Biol Phys 49:1097–1108

    PubMed  CAS  Google Scholar 

  14. Mason RP, Antich PP, Babcock EE, Constantinescu A, Peschke P, Hahn EW (1994) Non-invasive determination of tumor oxygen tension and local variation with growth. Int J Radiat Oncol Biol Phys 29:95–103

    PubMed  CAS  Google Scholar 

  15. Baldwin NJ, Ng TC (1996) Oxygenation and metabolic status of KHT tumors as measured simultaneously by 19F magnetic resonance imaging and 31P magnetic resonance spectroscopy. Magn Reson Imaging 14:541–551

    PubMed  CAS  Google Scholar 

  16. Dardzinski BJ, Sotak CH (1994) Rapid tissue oxygen tension mapping using 19F inversion-recovery echo-planar imaging of perfluoro-15-crown-5-ether. Magn Reson Med 32:88–97

    PubMed  CAS  Google Scholar 

  17. van der Sanden BP, Heerschap A, Hoofd L, Simonetti AW, Nicolay K, van der Toorn A, et al. (1999) Effect of carbogen breathing on the physiological profile of human glioma xenografts. Magn Reson Med 42:490–499

    Article  PubMed  Google Scholar 

  18. Evelhoch JL, Ackerman JJH (1983) NMR T1 measurements in inhomogeneous B1 with surface coils. J Magn Reson 53:52–64

    CAS  Google Scholar 

  19. Bewick V, Cheek L, Ball J (2003) Statistics review 7: Correlation and regression. Crit Care 7:451–459

    PubMed  Google Scholar 

  20. Ethofer T, Mader I, Seeger U, Helms G, Erb M, Grodd W, et al. (2003) Comparison of longitudinal metabolite relaxation times in different regions of the human brain at 1.5 and 3 Tesla. Magn Reson Med 50:1296–1301

    Article  PubMed  CAS  Google Scholar 

  21. Mason RP, Antich PP, Babcock EE, Gerberich JL, Nunnally RL (1989) Perfluorocarbon imaging in vivo: a 19F MRI study in tumor-bearing mice. Magn Reson Imaging 7:475–485

    PubMed  CAS  Google Scholar 

  22. Milross CG, Tucker SL, Mason KA, Hunter NR, Peters LJ, Milas L (1997) The effect of tumor size on necrosis and polarographically measured pO2. Acta Oncol 36:183–189

    PubMed  CAS  Google Scholar 

  23. Khalil AA, Horsman MR, Overgaard J (1995) The importance of determining necrotic fraction when studying the effect of tumour volume on tissue oxygenation. Acta Oncol 34:297–300

    PubMed  CAS  Google Scholar 

  24. Kozlowski P, Wong J, Goldenberg SL. Serial tumour blood flow measurements in the androgen dependent and androgen independent shionogi tumour model. Br J Urol (in press)

  25. Nordsmark M, Bentzen S, Overgaard J (1994) Measurement of human tumor oxygenation status by a polarographic needle electrode. Acta Oncol 33:383–389

    CAS  PubMed  Google Scholar 

  26. Brizel DM, Rosner GL, Proznitz LR, Dewhirst MW (1995) Patterns and variability of tumor oxygenation in soft tissue sarcomas, cervical sarcomas, and lymph nodes metastases. Int J Radiat Oncol Biol Phys 32:1121–1125

    PubMed  CAS  Google Scholar 

  27. Jaeger KD, Merlo FM, Kavanagh MC, Fyles AW, Hedley D, Hill RP (1998) Heterogeneity of tumor oxygenation: relationship to tumor necrosis, tumor size, and metastasis. Int J Radiat Oncol Biol Phys 42:717–721

    PubMed  Google Scholar 

  28. Jain RK, Safabakhsh N, Sckell A, Chen Y, Jiang P, Benjamin L, et al. (1998) Endothelial cell death, angiogenesis, and microvascular function after castration in an androgen-dependent tumor: role of vascular endothelial growth factor. Proc Natl Acad Sci USA 95:10820–10825

    PubMed  CAS  Google Scholar 

  29. Hansen-Algenstaedt N, Stoll BR, Padera TP, Dolmans DE, Hicklin DJ, Fukumura D, et al. (2000) Tumor oxygenation in hormone-dependent tumors during vascular endothelial growth factor receptor-2 blockade, hormone ablation, and chemotherapy. Cancer Res 60:4556–4560

    PubMed  CAS  Google Scholar 

  30. Skov K, Adomat H, Bowden M, Dragowska W, Gleave ME, Koch CJ et al. (2004) Hypoxia in the androgen-dependent shionogi model for prostate cancer at three stages. Radiat Res (in press)

  31. Koch CJ (2002) Measurement of absolute oxygen levels in cells and tissues using oxygen sensors and 2-nitroimidazole EF5. Methods Enzymol 352:3–31

    Article  PubMed  CAS  Google Scholar 

  32. Horsman MR, Khalil AA, Nordsmark M, Grau C, Overgaard J (1993) Relationship between radiobiological hypoxia and direct estimates of tumour oxygenation in a mouse tumour model. Radiother Oncol 28:69–71

    PubMed  CAS  Google Scholar 

  33. Horsman MR, Khalil AA, Siemann DW, Grau C, Hill SA, Lynch EM, et al. (1994) Relationship between radiobiological hypoxia in tumors and electrode measurements of tumor oxygenation. Int J Radiat Oncol Biol Phys 29:439–442

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments.

The authors thank Ms. Mary Bowden from the Prostate Centre at VGH for her excellent technical support. Generous help of Fresenius-Kabi, Clayton, NC, USA in the preparation of the PFC emulsion is gratefully acknowledged. This work was supported by research grants from Health Canada and Terry Fox Foundation.

Author information

Authors and Affiliations

  1. The Prostate Centre at VGH, 2660 Oak Street, Vancouver, BC, Canada, V6H 3Z6

    J. A. McNab, A. C. Yung & P. Kozlowski

  2. Department of Surgery, University of British Columbia, Vancouver, BC, Canada

    P. Kozlowski

Authors
  1. J. A. McNab
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. C. Yung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Kozlowski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. Kozlowski.

Rights and permissions

Reprints and permissions

About this article

Cite this article

McNab, J., Yung, A. & Kozlowski, P. Tissue oxygen tension measurements in the Shionogi model of prostate cancer using 19F MRS and MRI. MAGMA 17, 288–295 (2004). https://doi.org/10.1007/s10334-004-0083-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10334-004-0083-3

Keywords

Search

Navigation