Abstract
Japanese apricot (Prunus mume Sieb. et Zucc.) is one of the most popular fruit tree species in Japan and is most densely cultivated in Wakayama Prefecture. Since the 1980s, graft-transmissible symptoms, such as leaf-edge necrosis and incomplete flower development, have been observed in Nanko, the major Japanese apricot cultivar in Wakayama. Two ampeloviruses, plum bark necrosis stem pitting-associated virus (PBNSPaV) and little cherry virus 2 (LChV-2) were detected in Nanko trees showing typical symptoms. However, there is currently insufficient epidemiological data to infer that these viruses cause the symptoms. In this study, we conducted a large-scale survey of symptoms and RT-PCR detection of the two viruses in midwestern districts, the major cultivation areas in Wakayama. Among 208 surveyed Nanko trees, two viral infections were significantly associated with incomplete flower development, low fruit bearing rate, and interveinal chlorosis. LChV-2 infection also affected Nanko fruit and stone sizes. Additionally, we showed that the two viruses were already widely distributed throughout the main cultivation area. These results imply that the two ampeloviruses cause overall yield loss in Japanese apricot in Wakayama Prefecture.
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References
Bajet NB, Unruh TR, Druffel KL, Eastwell KC (2008) Occurrence of two little cherry viruses in sweet cherry in Washington State. Plant Dis 92:234–238
Bortiri E, Oh SH, Jiang J, Baggett S, Granger A, Weeks C, Buckingham M, Potter D, Parfitt DE (2001) Phylogeny and systematics of Prunus (Rosaceae) as determined by sequence analysis of ITS and the chloroplast trnL-trnF spacer DNA. Syst Bot 26:797–807
Iemura H, Shimazu K, Natsumi K (1995) Occurrence of a graft-transmissible disease of mume (Abstract in Japanese). Ann Phytopathol Soc Jpn 61:631
Jelkmann W, Eastwell KC (2011) Little cherry virus-1 and – 2. In: Hadidi A, Barba M, Candresse T, Jelkmann W (eds) Virus and virus-like diseases of pome and stone fruits. APS Press, St. Paul, pp 153–159
Kansako M, Iemura H, Natsumi K, Shimazu K (2000) Studies on Japanese apricot [Prunus mume] leaf-edge necrosis disease. Bull Wakayama Res Center Agric For Fish 1:67–78 (in Japanese with English summary)
Kurihara J, Tomaru K, Otsubo T, Natsuaki KT, Arimoto Y (2006) Mume leaf margin necrosis, a new disease of Japanese apricot [Prunus mume] caused by multiple infections with Cucumber mosaic virus and prunus necrotic ringspot related Ilarvirus. J Agri Sci Tokyo Univ Agric 50:95–105
Maejima K, Himeno M, Netsu O, Ishikawa K, Yoshida T, Fujita N, Hashimoto M, Komatsu K, Yamaji Y, Namba S (2014) Development of an on-site plum pox virus detection kit based on immunochromatography. J Gen Plant Pathol 80:176–183
MAFF (2016) Statistical tables, VI, Agricultural land, VII Crops, 6 fruits and nuts, (1) major fruits and nuts. In: The 90th Statistical yearbook of Ministry of Agriculture, Forestry and Fisheries. Available http://www.maff.go.jp/e/data/stat/90th/index.html#7. Accessed 25 Nov 2017
Marini DB, Zhang YP, Rowhani A, Uyemoto JK (2002) Etiology and host range of a Closterovirus associated with plum bark necrosis-stem pitting disease. Plant Dis 86:415–417
Mega K, Tomita E, Iwasa R, Kitamura S, Kato Y, Saito S, Mizukami S (1988) Ume (In Japanese). In: Aoba T et al (eds) The grand dictionary of horticulture, vol 1. Shogakukan, Tokyo, pp 289–300
Mekuria TA, Smith TJ, Beers E, Eastwell KC (2013) Little cherry virus 2 is transmitted to sweet cherry by Pseudococcus maritimus (Ehrhorn), a new vector of this virus. Plant Dis 97:851
Nakaune R, Nakano M (2006) Efficient methods for sample processing and cDNA synthesis by RT-PCR for the detection of grapevine viruses and viroids. J Virol Methods 134:244–249
Nakaune R, Takeda T, Numaguchi K, Nakazono-Nagaoka E, Fujikawa T (2018) Ampeloviruses associated with incomplete flower syndrome and leaf-edge necrosis in Japanese apricot. J Gen Plant Pathol 84:202–207
Ohtubo T, Kurihara J, Sakakibara M, Tomaru K (2002) Confirmation of symptom reproduction in mume leaf margin necrosis disease on Prunus mume (cv. Nankou) by sap inoculation with causal two viruses, Cucumber mosaic and Prunus necrotic ringspot-like viruses. J Agri Sci Tokyo Univ Agric 47:11–16
Otsubo T, Harano H, Natuaki K, Tomaru K (1991) Chakasu disorder of Japanese apricot (in Japanese). J Jpn Soc Hortic Sci 60:164–165
R Development Core Team (2008) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0
Raine J, McMullen RD, Forbes RD (1986) Transmission of the agent causing little cherry disease by the apple mealybug Phenacoccus aceris and the dodder Cuscuta lupuliformis. Can J Plant Pathol 8:6–11
Rott ME, Jelkmann W (2001) Detection and partial characterization of a second closterovirus associated with little cherry disease, Little cherry virus-2. Phytopathology 91:261–267
Shimazu K, Natsumi K (1989) Tests of causal factors of ‘mume chagasu’ syndrome (in Japanese). Ann Rep Wakayama Fruit Tree Exp Sta 114–115
Uyemoto JK, Teviotdale BL (1996) Graft-transmission of the causal agent of a bark necrosis-stem pitting disease of Black Beauty plum (Prunus salicina). Phytopathology 86:111–112
Yaegaki H (2013) Production, marketing, processing and breeding objective of Japanese apricot. Bull NARO Instit Fruit Tree Sci 16:1–12 (in Japanese with English synopsis)
Acknowledgements
We thank Mr. T. Yamanouchi, JA-kisyu, Mr. Y. Enomoto, JA-kinan, and Mr. Y. Taira, Minabe town for arranging the orchard visits and Mr. Y. Akagi, Mr. H. Hashimoto, Mr. M. Kawamura, Mr. S. Sahara, Mr. Y. Tabata, and Mr. T. Tanaka, Wakayama Prefecture, for helping with the field investigation. We profusely thank Mr. T. Tanimoto and Mr. A. Okada for providing fruits for study. This work was financially supported by the National Agriculture and Food Research Organization (NARO) and Wakayama Prefecture.
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Numaguchi, K., Takeda, T., Tsuchida, Y. et al. Large-scale field survey reveals overall yield loss in Japanese apricot possibly caused by two ampeloviruses. J Gen Plant Pathol 85, 116–121 (2019). https://doi.org/10.1007/s10327-018-0831-2
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DOI: https://doi.org/10.1007/s10327-018-0831-2