Abstract
Cucurbit chlorotic yellows virus (CCYV), a whitefly-transmitted virus, is a new member of the Crinivirus genus, that causes major economic losses in cucumber, melon and watermelon crops. To develop immunodiagnostic methods for CCYV, we produced an antiserum by immunizing rabbits with bacterially expressed recombinant coat protein of CCYV and detected CCYV from CCYV-infected plants with western blotting and immunoelectron microscopy. CCYV in extracts from infected plants was also readily detected by double antibody sandwich enzyme-linked immunosorbent assay with high sensitivity and specificity. A tissue blot immunoassay indicated that CCYV localizes in the phloem tissues of the petiole and is distributed in tiny spots within the leaf lamina.
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Abbreviations
- CCYV:
-
Cucurbit chlorotic yellows virus
- CP:
-
Coat protein
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Acknowledgments
We are grateful to Dr. Masahiro Nao of Ehime Prefecture Agricultural Experiment Station, Japan for the kind gift of BPYV-infected cucumber plants and Dr. Mitsuru Okuda of National Agricultural Research Center for Kyushu Okinawa Region, Japan for providing RNA from CCYV-infected cucumber plants. This work was supported in part by a grant in aid, research project for utilizing advanced technologies in agriculture, forestry and fisheries, from the Ministry of Agriculture, Forestry and Fisheries of Japan.
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Kubota, K., Usugi, T. & Tsuda, S. Production of antiserum and immunodetection of Cucurbit chlorotic yellows virus, a novel whitefly-transmitted crinivirus. J Gen Plant Pathol 77, 116–120 (2011). https://doi.org/10.1007/s10327-010-0287-5
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DOI: https://doi.org/10.1007/s10327-010-0287-5