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Role of macrophage polarization in periodontitis promoting atherosclerosis

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Abstract

Periodontitis is a chronic inflammatory destructive disease occurring in periodontal supporting tissues. Atherosclerosis(AS) is one of the most common cardiovascular diseases. Periodontitis can promote the development and progression of AS. Macrophage polarization is closely related to the development and progression of the above two diseases, respectively. The purpose of this animal study was to evaluate the effect of periodontitis on aortic lesions in atherosclerotic mice and the role of macrophage polarization in this process. 45 ApoE-/-male mice were randomly divided into three groups: control (NC), atherosclerosis (AS), and atherosclerosis with periodontitis (AS + PD). Micro CT, serological testing and pathological testing(hematoxylin–eosin staining, oil red O staining and Masson staining) were used for Evaluate the modeling situation. Immunohistochemistry(IHC) and immunofluorescence(IF) were performed to evaluate macrophage content and macrophage polarization in plaques. Cytokines associated with macrophage polarization were analyzed using quantitative real-time polymerase chain reaction(qRT-PCR) and enzyme-linked immunosorbent assay(Elisa). The expression of macrophages in plaques was sequentially elevated in the NC, AS, and AS + PD groups(P < 0.001). The expression of M1 and M1-related cytokines showed the same trend(P < 0.05). The expression of M2 and M2-related cytokines showed the opposite trend(P < 0.05). The rate of M1/M2 showed that AS + PD > AS > NC. Our preliminary data support that experimental periodontitis can increase the content of macrophage in aortic plaques to exacerbate AS. Meanwhile, experimental periodontitis can increase M1 macrophages, and decrease M2 macrophages, increasing M1/M2 in the plaque.

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Data available on request from the authors.

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Acknowledgements

This study was supported by grants from the Beijing-Tianjin-Hebei Region's Medical and Health Coordinated Development and Public HospitalNoReform Capacity Building of China (2019061636).

Funding

Beijing-Tianjin-Hebei Region’s Medical and Health Coordinated Development and Public HospitalNoReform Capacity Building of China,2019061636,zhe ma

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Authors and Affiliations

  1. Department of Preventive Dentistry, Hebei Key Laboratory of Stomatology, Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, No.383, Zhongshan East Road, Changan District, Shijiazhuang, Hebei, China

    Mingyue Shi, Kaili Guo, Yue Liu, Fengdi Cao, Tiantian Fan, Zhuohang Deng, Yuhan Meng, Mingyang Bu & Zhe Ma

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  1. Mingyue Shi
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Shi, M., Guo, K., Liu, Y. et al. Role of macrophage polarization in periodontitis promoting atherosclerosis. Odontology (2024). https://doi.org/10.1007/s10266-024-00935-z

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