Skip to main content

Advertisement

SpringerLink
Log in

Peri-implant tissue augmentation by volume-stable collagen matrix transplantation: a study of dog mandibles

  • Original Article
  • Published:
Odontology Aims and scope Submit manuscript

Abstract

The aim of this study was to investigate histologically the amount of peri-implant tissue augmentation after volume-stable porcine collagen matrix transplantation. Six male beagle dogs were used in the experiment. P2, P4, and M1 distal roots were extracted under general anesthesia. After 6 months, implants were placed in the same sites, and volume-stable porcine collagen matrix transplantation was performed. Impressions were taken at 1 and 2 weeks and at 1, 2, and 3 months after transplantation. The dogs were euthanized at 3 months, and their mandibles were removed and scanned using micro-computed tomography. Standard Triangulated Language data were also obtained. Using preoperative models as a reference, the data for all time points were compared, and changes in the thickness of the cross-section of the implant sites were measured. The model created at 3 months was then compared with the mandible data, and the thickness of collected peri-implant soft tissue was measured under optical microscopy. Increased thickness was found at some of the sites on the buccal side. Regarding the peri-implant soft tissue, the thickness of the measured sites on the buccal side was significantly increased at 3 months in the experimental group. Histological observations of the internal structures of the tissue in the experimental group revealed irregular collagen fibers and a remnant collagen matrix. Endogenous tissue was observed within the collagen matrix, indicating good fusion with the surrounding autologous tissue. These results suggest that volume-stable porcine collagen matrix transplantation promotes peri-implant tissue augmentation on the buccal side.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Araújo MG, Sukekava F, Wennström JL, Lindhe J. Ridge alterations following implant placement in fresh extraction sockets: an experimental study in the dog. J Clin Periodontol. 2005;32:645–52.

    Article  Google Scholar 

  2. Tan WL, Wong TL, Wong MC, Lang NP. A systematic review of post-extractional alveolar hard and soft tissue dimensional changes in humans. Clin Oral Implants Res. 2012;23:1–21.

    Article  Google Scholar 

  3. Jung RE, Holderegger C, Sailer I, Khraisat A, Suter A, Hämmerle CHF. The effect of all-ceramic and porcelain-fused-to-metal restorations on marginal peri-implant soft tissue color: a randomized controlled clinical trial. Int J Periodontics Restorative Dent. 2008;28:357–65.

    PubMed  Google Scholar 

  4. Briguglio F, Falcomatà D, Marconcini S, Fiorillo L, Briguglio R, Farronato D. The use of titanium mesh in guided bone regeneration: a systematic review. Int J Dent. 2019; 2019: 9065423.

  5. Thoma DS, Benic GI, Zwahlen M, Hämmerle CHF, Jung RE. A systematic review assessing soft tissue augmentation techniques. Clin Oral Implants Res. 2009;20:146–65.

    Article  Google Scholar 

  6. Cairo F, Barbato L, Tonelli P, Batalocco G, Pagavino G, Nieri M. Xenogeneic collagen matrix versus connective tissue graft for buccal soft tissue augmentation at implant site. A randomized, controlled clinical trial. J Clin Periodontol. 2017;44:769–76.

    Article  Google Scholar 

  7. Kinsel RP, Capoferri D. Simplified method to develop optimal gingival contours for the single implant-supported, metal-ceramic crown in the aesthetic zone. Pract Proced Aesthet Dent. 2008;20:231–6.

    PubMed  Google Scholar 

  8. Sanz M, Simion M, Working Group 3 of the European Workshop on Periodontology. Surgical techniques on periodontal plastic surgery and soft tissue regeneration: Consensus Report of Group 3 of the 10th European Workshop on Periodontology. J Clin Periodontol. 2014;41:S92–7.

    Article  Google Scholar 

  9. Thoma DS, Buranawat B, Hämmerle CHF, Held U, Jung RE. Efficacy of soft tissue augmentation around dental implants and in partially edentulous areas: a systematic review. J Clin Periodontol. 2014;41:S77–91.

    Article  Google Scholar 

  10. Rossmann JA, Rees TD. A comparative evaluation of hemostatic agents in the management of soft tissue graft donor site bleeding. J Periodontol. 1999;70:1369–75.

    Article  Google Scholar 

  11. Del Pizzo M, Modica F, Bethaz N, Priotto P, Romagnoli R. The connective tissue graft: a comparative clinical evaluation of wound healing at the palatal donor site. A preliminary study. J Clin Periodontol. 2002;29:848–54.

    Article  Google Scholar 

  12. Soileau KM, Brannon RB. A histologic evaluation of various stages of palatal healing following subepithelial connective tissue grafting procedures: a comparison of eight cases. J Periodontol. 2006;77:1267–73.

    Article  Google Scholar 

  13. Griffin TJ, Cheung WS, Zavras AI, Damoulis PD. Postoperative complications following gingival augmentation procedures. J Periodontol. 2006;77(12):2070–9.

    Article  Google Scholar 

  14. Thoma DS, Nänni N, Benic GI, Weber FE, Hämmerle CHF, Jung RE. Effect of platelet-derived growth factor-BB on tissue integration of cross-linked and non-cross-linked collagen matrices in a rat ectopic model. Clin Oral Implants Res. 2015;26(3):263–70.

    Article  Google Scholar 

  15. Thoma DS, Hämmerle CH, Cochran DL, Jones AA, Görlach C, Uebersax L, Mathes S, Graf-Hausner U, Jung RE. Soft tissue volume augmentation by the use of collagen-based matrices in the dog mandible—a histological analysis. J Clin Periodontol. 2011;38:1063–70.

    Article  Google Scholar 

  16. Willershausen I, Barbeck M, Boehm N, Sader R, Willershausen B, Kirkpatrick CJ, Ghanaati S. Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence. J Appl Oral Sci. 2014;22:29–37.

    Article  Google Scholar 

  17. Pabst AM, Happe A, Callaway A, Ziebart T, Stratul SI, Ackermann M, Konerding MA, Willershausen B, Kasaj A. In vitro and in vivo characterization of porcine acellular dermal matrix for gingival augmentation procedures. J Periodontal Res. 2014;49:371–81.

    Article  Google Scholar 

  18. McGuire MK, Scheyer ET. Xenogeneic collagen matrix with coronally advanced flap compared to connective tissue with coronally advanced flap for the treatment of dehiscence-type recession defects. J Periodontol. 2010;81:1108–17.

    Article  Google Scholar 

  19. Sanz M, Lorenzo R, Aranda JJ, Martin C, Orsini M. Clinical evaluation of a new collagen matrix (Mucografts prototype) to enhance the width of keratinized tissue in patients with fixed prosthetic restorations: a randomized prospective clinical trial. J Clin Periodontol. 2009;36:868–76.

    Article  Google Scholar 

  20. Thoma DS, Zeltner M, Hilbe M, Hämmerle CHF, Hüsler J, Jung RE. Randomized controlled clinical study evaluating effectiveness and safety of a volume-stable collagen matrix compared to autogenous connective tissue grafts for soft tissue augmentation at implant sites. J Clin Periodontol. 2016;43:874–85.

    Article  Google Scholar 

  21. Huber S, Zeltner M, Hämmerle CHF, Jung RE, Thoma DS. Non-interventional 1-year follow-up study of peri-implant soft tissues following previous soft tissue augmentation and crown insertion in single-tooth gaps. J Clin Periodontol. 2018;45:504–12.

    Article  Google Scholar 

  22. Thoma DS, Naenni N, Benic GI, Hämmerle CHF, Jung RE. Soft tissue volume augmentation at dental implant sites using a volume stable three-dimensional collagen matrix—histologic outcomes of a preclinical study. J Clin Periodontol. 2017;44:185–94.

    Article  Google Scholar 

  23. Seo GY, Thoma DS, Jung UW, Lee JS. Increasing the tissue thickness at implant sites using guided bone regeneration and an additional collagen matrix: histologic observations in beagle dogs. J Biomed Mater Res B Appl Biomater. 2019;107:741–9.

    Article  Google Scholar 

  24. Schmitt CM, Matta RE, Moest T, Humann J, Gammel L, Neukam FW, Schlegel KA. Soft tissue volume alterations after connective tissue grafting at teeth: the subepithelial autologous connective tissue graft versus a porcine collagen matrix—a pre-clinical volumetric analysis. J Clin Periodontol. 2016;43:609–17.

    Article  Google Scholar 

  25. Naenni N, Bienz SP, Benic GI, Jung RE, Hämmerle CHF, Thoma DS. Volumetric and linear changes at dental implants following grafting with volume-stable three-dimensional collagen matrices or autogenous connective tissue grafts: 6-month data. Clin Oral Investig. 2018;22:1185–95.

    Article  Google Scholar 

  26. Mathes SH, Wohlwend L, Uebersax L, Mentlen VR, Thoma DS, Jung RE, Görlach C, Graf-Hausner U. A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts. Biotechnol Bioeng. 2010;107:1029–39.

    Article  Google Scholar 

  27. Thoma DS, Jung RE, Schneider D, Cochran DL, Ender A, Jones AA, Görlach C, Uebersax L, Graf-Hausner U, Hämmerle CH. Soft tissue volume augmentation by the use of collagen-based matrices: a volumetric analysis. J Clin Periodontol. 2010;37:659–66.

    Article  Google Scholar 

  28. Rothamel D, Benner M, Fienitz T, Happe A, Kreppel M, Nickenig HJ, Zöller JE. Biodegradation pattern and tissue integration of native and cross-linked porcine collagen soft tissue augmentation matrices—an experimental study in the rat. Head Face Med. 2014;10:10.

    Article  Google Scholar 

  29. Schwarz F, Rothamel D, Herten M, Sager M, Becker J. Angiogenesis pattern of native and cross-linked collagen membranes: an immunohistochemical study in the rat. Clin Oral Implants Res. 2006;17:403–9.

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to express their heartfelt thanks to Professor Mikio Ishiyama of the Second Department of Anatomy, Nippon Dental University School of Life Dentistry at Niigata, for his guidance and cooperation in regard to this study. The authors would also like to thank Shofu, Inc. (Kyoto, Japan) for providing the specimens.

Author information

Authors and Affiliations

  1. Department of Crown and Bridge Prosthodontics, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamauracho, Chuo-ku, Niigata, 951-8580, Japan

    Atsuo Sasagawa, Kazuhiko Ueda & Fumihiko Watanabe

  2. Department of Dental Materials Science, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamauracho, Chuo-ku, Niigata, 951-8580, Japan

    Kensuke Igarashi

  3. Oral Implant Care Unit, Niigata Hospital, The Nippon Dental University, 1-8 Hamauracho, Chuo-ku, Niigata, 951-8580, Japan

    Kazuhiko Hiroyasu

Authors
  1. Atsuo Sasagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kensuke Igarashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kazuhiko Ueda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kazuhiko Hiroyasu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fumihiko Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Atsuo Sasagawa.

Ethics declarations

conflict of interest

The authors declare no conflicts of interest associated with this manuscript.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sasagawa, A., Igarashi, K., Ueda, K. et al. Peri-implant tissue augmentation by volume-stable collagen matrix transplantation: a study of dog mandibles. Odontology 110, 81–91 (2022). https://doi.org/10.1007/s10266-021-00639-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10266-021-00639-8

Keywords

Search

Navigation