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Three-dimensional observation of mouse tongue muscles using micro-computed tomography

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Abstract

The aim of this study is to obtain information about the mouse tongue muscle rendered using micro-computed tomography (μCT) at low, middle, and high magnifications. Three-dimensional (3D) μCT is used in various fields. Most μCT observations are restricted to hard tissue in biomaterial samples. Recently, with the use of osmium tetroxide, μCT has been effectively employed to observe soft tissue; it is now believed that μCT observation of soft tissue is feasible. On the other hand, the structure of the tongue muscle has been well studied, but cross-sectional imaging enhanced by 3D rendering is lacking. We chose the mouse tongue as a soft tissue case study for μCT and generated cross-sectional images of the tongue enhanced by 3-D image rendering with histological resolution. During this observation, we developed new methods of low-magnification observation to show the relation between the tongue muscles and surrounding tissues. We also applied high-resolution μCT in high-magnification observation of muscle fiber fascicles. Our methodological techniques give the following results: (1) For low-magnification observation (field of view: 12,000 μm), pretreatment with decalcification and freeze drying is suitable for observing the area between the muscle of the tongue and the bone around the tongue using μCT. (2) For middle-magnification observation (Field of view: 3,500 μm), the use of osmium tetroxide to observe the muscle arrangement of the tongue by μCT is suitable. (3) For high-magnification observation (Field of view: 450 μm), high-resolution μCT is suitable for observation of the transversus muscle fiber fascicles.

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Acknowledgments

We are grateful to Prof. M. Tsuchimochi of the Department of Oral and Maxillofacial Radiology of the Nippon Dental University School of Life Dentistry at Niigata for his encouragement and constant support. We also wish to thank Prof. A. Ezura of General Dentistry at the Nippon Dental University School of Life Dentistry at Niigata for his help and suggestions. This research was supported by Research Promotion Grants (No. NDUF-12-07) from the Nippon Dental University.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-chou, Chuo-ku, Niigata, 951-8580, Japan

    Hidekazu Aoyagi & Kenzirou Nakamura

  2. Department of Physiology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-chou, Chuo-ku, Niigata, 951-8580, Japan

    Shin-ichi Iwasaki

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  1. Hidekazu Aoyagi
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  2. Shin-ichi Iwasaki
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Correspondence to Hidekazu Aoyagi.

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Aoyagi, H., Iwasaki, Si. & Nakamura, K. Three-dimensional observation of mouse tongue muscles using micro-computed tomography. Odontology 103, 1–8 (2015). https://doi.org/10.1007/s10266-013-0131-4

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  • DOI: https://doi.org/10.1007/s10266-013-0131-4

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