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Molecular systematics of Angelica and allied genera (Apiaceae) from the Hengduan Mountains of China based on nrDNA ITS sequences: phylogenetic affinities and biogeographic implications

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Abstract

Maximum parsimony, maximum likelihood, and Bayesian analyses of nuclear ribosomal DNA internal transcribed spacer sequences were used to infer the phylogenetic affinities and historical biogeography of Angelica and its allies (Apiaceae tribe Selineae), with emphasis on those species of Angelica and Peucedanum endemic to the Hengduan Mountains of south-central China. Results of these analyses corroborate a monophyletic Angelica (Angelica sensu stricto) upon the inclusion of Coelopleurum, Czernaevia, and one of two examined species of Ostericum, but with the exclusion of several species previously attributable to Angelica. Angelica oncosepala and A. likiangensis arise within the genus Heracleum in tribe Tordylieae; the former is recognized under its original name, Heracleum oncosepalum. Angelica sinensis, A. tianmuensis and A. paeoniifolia arise within the Sinodielsia clade of previous circumscription, closely related to Levisticum officinale. Angelica anomala is a sister group to Ostericum grosseserratum in the previously delimited Acronema clade. Angelica apaensis and A. decursiva, taxa whose phylogenetic affinities have previously been controversial, are confirmed within Angelica. Northeast Asia (including Japan, northeast China, Korea and adjacent areas of Russia), Western Europe, and North America are inferred to be ancestral areas of Angelica based on optimal solutions of a dispersal-vicariance analysis, with the Hengduan Mountains likely providing a refugium for Angelica during the latter part of the Tertiary.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China, Grant No. 30670146, and the National Infrastructure of Natural Resources for Science and Technology, Grant No. 2005DKA21403. The authors greatly appreciate Dr. Kun Wei for comments on an early draft of the manuscript and one anonymous reviewer for helpful criticism.

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Authors and Affiliations

  1. Laboratory of Systematic and Evolutionary Botany, College of Life Sciences, Sichuan University, Wangjiang Road #29, 610064, Chengdu, Sichuan, People’s Republic of China

    Tu Feng, Yan Yu, Xuemei Zhang, Weiwei Chen, Xingjin He & Shuang Liu

  2. Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    Stephen R. Downie

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  1. Tu Feng
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  3. Yan Yu
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Correspondence to Xingjin He.

Appendix

Appendix

Taxa, references, and GenBank accession numbers for all nrDNA ITS sequences used in this study.

Angelica acutiloba (Siebold & Zuccarini) Kitagawa, AJ131291, GenBank; A. ampla A. Nelson, U79597, U79598, Downie et al. 1998; A. amurensis Schischkin in Schischkin & Bobrov, DQ263581, Xue et al. 2007; A. anomala Avé-Lallemant in Fischer & Avé-Lallemant, DQ263582, GenBank; A. apaensis R. H. Shan & C. Q. Yuan, EU418381, N: 31°54.211′, E: 102°39.723′, this study; DQ263583, Xue et al. 2007, endemic in Hengduan Mountains; A. archangelica L., U30576, U30577, Downie et al. 1998; A. arguta Nutt. ex Torr. & A. Gray, U79599, U79600, Downie et al. 1998; A. biserrata C. Q. Yuan & R. H. Shan, DQ270207, Xue et al. 2007; A. breweri A. Gray, U78396, U78456, Downie et al. 1998; A. cartilaginomarginata var. foliosa C. Q. Yuan & R. H. Shan, DQ263589, GenBank; A. cincta H. Boissieu, AF008601, AF009080, Katz-Downie et al. 1999; A. dahurica (Fischer ex Hoffmann) Bentham & J. D. Hooker ex Franchet & Savatier, EU418374, N: 25°04.106′, E: 102°42.212′, this study; A. decurrens (Ledeb.) B. Fedtsch., AF008599, AF009078, Katz-Downie et al. 1999; A. decursiva (Miquel) Franchet & Savatier, EU418375, N: 25°04.202′, E: 102°42.198′, this study; A. fargesii H. de Boissieu, EU418376, N: 31°48.514′, E: 108°46.539′, this study, endemic in Hengduan Mountains; A. furcijuga Kitagawa, DQ278164, Xue et al. 2007; A. genuflexa Nuttall., DQ263566, Xue et al. 2007; A. gigas Nakai, DQ263580, Xue et al. 2007; A. grayi (Coult. & Rose) Coult. & Rose., AY146825, AY146891, Sun et al. 2004; A. japonica A. Gray, DQ278166, Xue et al. 2007; A. kangdingensis R. H. Shan & F. D. Pu, DQ263584, Xue et al. 2007, endemic in Hengduan Mountains; A. keiskei (Miq.) Koidz., DQ263562, Xue et al. 2007; A. koreana Maxim. = (Ostericum grosseserratum Maxim.), AF455749, GenBank; A. laxifoliata Diels, EU647210, N: 30°03.543′, E: 101°48.881′, this study; DQ263586, Xue et al. 2007, endemic in Hengduan Mountains; A. lignescens Danton & Reduron, AY179030, Spalik et al. 2004; A. likiangensis H. Wolff, DQ263587, Xue et al. 2007, endemic in Hengduan Mountains; A. maowenensis C. Q. Yuan & R. H. Shan, DQ263585, Xue et al. 2007; EU236157, Zhou et al. 2008, endemic in Hengduan Mountains; A. megaphylla Diels, EU418377, N: 29°05.200′, E: 107°15.114′, this study, endemic in Hengduan Mountains; A. morii Hayata, DQ263578, Xue et al. 2007; A. nitida H. Wolff, EU418378, N: 32°44.529′, E: 103°42.045′, this study, endemic in Hengduan Mountains; A. omeiensis C. C. Yuan & R. H. Shan., DQ263576, Xue et al. 2007, endemic in Hengduan Mountains; A. oncosepala Handel-Mazzetti, EU418382, N: 27°06.774′, E: 100°11.322′, this study, endemic in Hengduan Mountains; A. paeoniifolia C. Q. Yuan & R. H. Shan, FJ237533, N:29°39.221′, E:94°32.803, this study, endemic in Hengduan Mountains; A. pinnata S. Watson, AF358465, AF358532, Downie et al. 2002; A. polymorpha Maxim., U78415, U78475, Downie et al. 1998; A. pseudoselinum H. de Boissieu, EU418379, N: 30°45.008′, E: 102°78.6022′, this study, endemic in Hengduan Mountains; A. pubescens (R. H. Shan & C. Q. Yuan) C. Q. Yuan & R. H. Shan, DQ263567, Xue et al. 2007; A. purpureifolia Chuang, AY548229, GenBank; A. roseana Henderson, AF358466, AF358533, Downie et al. 2002; A. sachalinensis Maxim., U78413, U78473, Downie et al. 1998; A. sinensis (Oliver) Diels., FJ204235, N:30°41.452′; E:104°09.315′, this study, endemic in Hengduan Mountains; A. sylvestris Linnaeus, U78414, U78474, Downie et al. 1998; A. tianmuensis Z. H. Pan, & T. D. Zhuang, DQ270194, Xue et al. 2007; A. valida Diels, EU418380, N: 29°06.985′, E: 107°14.028′, this study, endemic in Hengduan Mountains; Coelopleurum lucidum (L.) Fernald, DQ270196, Xue et al. 2007; C. saxatile (Turcz. ex Ledeb.) Drude, DQ270195, Xue et al. 2007; Czernaevia laevigata Turcz., DQ270197, Xue et al. 2007; Heracleum aconitifolium Woronow, AF008625, AF009104, Katz-Downie et al. 1999; H. candicans Wall. ex DC., DQ516378, Spalik and Downie 2007; H. lanatum Michx., U30542, U30543, Downie and Katz-Downie 1996; H. moellendorffii Hance, AF164828, AF164853, Downie et al. 2000; H. rigens Wall. ex DC., U30548, U30549, Downie and Katz-Downie 1996; H. sphondylium L., U30544, U30545, Downie and Katz-Downie 1996; Levisticum officinale W. D. J. Koch, U78389, U78449, Downie et al. 1998; Ostericum grosseserratum (Maxim.) Kitaga., DQ270199, Xue et al. 2007; O. koreanum (Maxim.) Kitag., AY548212, GenBank; Peucedanum achaicum Halácsy. (= Holandrea achaia (Halácsy) Spalik, Reduron & S. R. Downie), AY164832, AY164857, Downie et al. 2000; P. bourgaei Lange, AF495818, AF495819, Spalik et al. 2004; P. caucasicum (M. Bieb.) K. Koch. (= Holandrea caucasica (M. Bieb.) Spalik, Reduron & S. R. Downie), AF008618, AF009097, Katz-Downie et al. 1999; P. coriaceum Rchb., AF495824, AF495825, Spalik et al. 2004; P. delavayi Franchet, EU418386, N: 26°95.016′, E: 100°00.459′, this study, endemic in Hengduan Mountains; P. dissolutum (Diels) H. Wolff, EU418388, N: 29°07.372′, E: 107°15.335′, this study, endemic in Hengduan Mountains; P. elegans Komarov, EU418385, N: 27°10.202′, E: 99°98.886′, this study; P. gallicum Latourr., AF495816, AF497817, Spalik et al. 2004; P. japonicum Thunberg ex A. Murray, AF495826, AF495827, Spalik et al. 2004; P. ledebourielloides Franch, DQ270200, Xue et al. 2007; P. morisonii Besser ex Schult., U78406, U78466, Downie et al. 1998; P. officinale L., AF495820, AF495821, Spalik et al. 2004; P. praeruptorum Dunn, EU418383, N: 31°50.005′, E: 108°60.141′, this study; P. pschavicum Boiss. (= Holandrea pschawica (Boiss.) Reduron, Charpin & Pimenov), AF008619, AF009098, Katz-Downie et al. 1999; P. rubricaule R. H. Shan & M. L. Sheh, EU418387, N: 27°42.403′, E: 100°09.251′, this study, endemic in Hengduan Mountains; P. terebinthaceum (Fisch. ex Trevis.) Fisch. ex Turez. (= Kitagawia terebinthacea (Fisch. ex Spreng.), AF164833, AF164858, Downie et al. 2000; P. zedelmeyerianum Manden., AF164834, AF164859, Downie et al. 2000; Pleurospermum decurrens Franchet, AF164837, AF164862, Downie et al. 2000; Pleurospermum foetens Franchet, AF008639, AF009118, Katz-Downie et al. 1999; Pleurospermum uralense Hoffmann, AF008638, AF009117, Katz-Downie et al. 1999.

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Feng, T., Downie, S.R., Yu, Y. et al. Molecular systematics of Angelica and allied genera (Apiaceae) from the Hengduan Mountains of China based on nrDNA ITS sequences: phylogenetic affinities and biogeographic implications. J Plant Res 122, 403–414 (2009). https://doi.org/10.1007/s10265-009-0238-4

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