Abstract
The use of insertion/deletion (indel) patterns from sequences of the trnL intron and trnL–F intergenic spacer (IGS) in finding plastid genome types of the genus Poa L. was studied. New sequences for 23 taxa (P. alpina, P. badensis, P. bulbosa, P. crassipes, P. molinerii, P. annua, P. chaixii, P. granitica, P. pratensis, P. sibirica, P. remota, P. botryoides, P. cenisia, P. compressa, P. laxa, P. margilicola, P. media, P. nemoralis, P. palustris, P. pannonica, P. pirinica, P. riphaea, and P. sejuncta) and 18 previously published sequences, which represent 11 of the 13 sections listed for Poa in Flora Europaea, were investigated. Collections were made primarily in central Europe. Indel patterns, despite sampling less than 0.7% of the plastid genome, produced four taxa groupings that were congruent with the major divisions obtained in intensive, previously published restriction-site studies. Insertion/deletion events in the trnL intron and trnL–trnF IGS were in nearly all cases unique to a single pattern group and thus provided almost no information about relationships among these groups. Indels did, however, provide a meaningful infrageneric classification criterion for Poa. They can serve as useful tools in studying relationships within this genus.
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Acknowledgements
The authors wish to thank R.J. Soreng for checking the determination of many of the specimens used in this study and for helpful comments; L.J. Gillespie for helpful comments and advice and for kindly providing a copy of the Gillespie and Soreng manuscript; T.R. Hodkinson and one anonymous reviewer for constructive comments; D. Bernátová, M. Burešová, M. Chytrý, V. Grulich, J.A. Holt, M. Kočí, I. Kuželová, S.L. Thatcher, C. Yankov, and L. Yankova for assisting with field collections; Y. Wang, R. Ma, and X. Chen for providing material from China; J.A. Holt for writing the computer program Invert and editing the manuscript; and J. Janeček and V. Černoch of the Plant Breeding Station Hladké Životice for providing the four commercial cultivars for sequencing. This research was funded by the Ministry of Education of the Czech Republic [material and sequencing with FRVŠ Project 556-G4 (Phylogenetic analysis of Poa L. on the basis of non-coding cpDNA sequences) and equipment with Research Project MSM 143100010 (Spatial and Temporal Biodiversity Dynamics in Ecosystems of Central Europe)]. This research was undertaken within the graduate studies of S.D.S.H., which were supported by a National Science Foundation Graduate Research Fellowship, a US Student Fulbright Grant, and an Honor Society of Phi Kappa Phi Fellowship.
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Stoneberg Holt, S.D., Horová, L. & Bureš, P. Indel patterns of the plastid DNA trnL–trnF region within the genus Poa (Poaceae). J Plant Res 117, 393–407 (2004). https://doi.org/10.1007/s10265-004-0172-4
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DOI: https://doi.org/10.1007/s10265-004-0172-4