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Macrophage chemotactic protein-1 mRNA levels in non-Hodgkin lymphoma

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Abstract

Non-Hodgkin lymphoma (NHL) is one of the most common malignancies whose incidence increases, and the treatment results are not satisfactory. The aim of this study was to determine the capacity of NHL to produce MCP-1, chemokine that induces chemotaxis of macrophages and lymphoid cells. The mRNA expression and protein MCP-1 expression were determined in the samples of 20 patients with NHL and 8 reactive tonsils. MCP-1 mRNA was detected in 8/8 tonsils and in 19/20 patients with NHL by real-time PCR analysis. In addition, the amount of detected MCP-1 cDNA was significantly higher in patients with limited stage, good IPI, normal level of fibrinogen and LDH. Finally, in patients with aggressive NHL, the level of MCP-1 cDNA was higher than in indolent tumours. Immunohistochemical analysis revealed that majority of stromal elements such as macrophages, endothelial and smooth muscle cells in reactive as well as in neoplastic lymphoid tissue showed strong cytoplasmic MCP-1 expression. Moderate cytoplasmic MCP-1 expression was also observed in reactive lymphocytes, while tumour cells of indolent NHL were mostly pale in comparison with aggressive lymphomas which predominantly demonstrated intense MCP-1 staining. These intriguing preliminary results emphasize the need for further investigations that must be conducted on the representative sample with concordant measurement of serum MCP-1 level.

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Acknowledgments

We thank our technicians Tanja Kovačević, Ozren Štanfel, Ankica Brajenić and Ivan Dašek for technical assistance. This work was supported by the Ministry of Science, Education and Sports of the Republic of Croatia (grant numbers 062-0620095-0082 and 062-0620095-0079).

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Authors and Affiliations

  1. Department of Haematology, Clinical Hospital Centre Rijeka, Rijeka, Croatia

    Toni Valković & Antica Duletić-Načinović

  2. Department of Pathology, School of Medicine, University of Rijeka, B.Branchetta 20, 51000, Rijeka, Croatia

    Sanja Štifter, Ita Hadžisejdić, Blaženka Grahovac & Nives Jonjić

  3. Department of Histology and Embriology, School of Medicine, University of Rijeka, Rijeka, Croatia

    Milena Hasan

  4. Department of Psychology, Faculty of Philosophy, University of Rijeka, Rijeka, Croatia

    David Zombori

Authors
  1. Toni Valković
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  2. Antica Duletić-Načinović
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  3. Sanja Štifter
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  4. Milena Hasan
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  5. Ita Hadžisejdić
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  7. Blaženka Grahovac
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  8. Nives Jonjić
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Correspondence to Nives Jonjić.

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Valković, T., Duletić-Načinović, A., Štifter, S. et al. Macrophage chemotactic protein-1 mRNA levels in non-Hodgkin lymphoma. Clin Exp Med 10, 229–235 (2010). https://doi.org/10.1007/s10238-010-0093-6

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  • DOI: https://doi.org/10.1007/s10238-010-0093-6

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