Abstract
Muscle fibers play a crucial role in the mechanical action of skeletal muscle tissue. However, it is unclear how the histological variations affect the mechanical properties of tissues. In this study, the shift of myosin heavy chain (MHC) isoforms is used for the first time to establish a linkage between tissue histological variation and passive mechanical properties. The shift of MHC isoform is found not only to induce significant differences in skeletal muscle passive mechanical properties, but also to lead to differences in strain rate responses. Non-negligible rate dependence is observed even in the conventionally defined quasi-static regime. Fidelity in the estimated constitutive parameters, which can be impacted due to variation in MHC isoforms and hence in rate sensitivity, is enhanced using a Bayesian inference framework. Subsequently, scanning electron microscopy and fluorescence microscopy are used to characterize the fracture morphology of muscle tissues and fibers. The fracture mode of both MHC I and II muscle fibers exhibited shearing of endomysium. Results show that the increase in strain rate only leads to stronger rebounding of the muscle fibers during tissue rupture without changing fracture modes.
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This work is supported by the National Natural Science Foundation of China (NNSFC) through Grants 12272132 and 11922206.
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Jiabao Tang involved in conceptualization, methodology, software, investigation, formal analysis, writing—original draft, and writing—review and editing. Wenyang Liu involved in conceptualization, methodology, formal analysis, writing—review and editing, and funding acquisition. Xuhong Li involved in conceptualization and writing—review and editing. Yun Peng involved in validation and writing—review and editing. Yingchun Zhang involved in writing—review and editing. Shujuan Hou involved in validation, writing—review and editing, and funding acquisition.
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Tang, J., Liu, W., Li, X. et al. Linking myosin heavy chain isoform shift to mechanical properties and fracture modes in skeletal muscle tissue. Biomech Model Mechanobiol 23, 103–116 (2024). https://doi.org/10.1007/s10237-023-01761-y
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DOI: https://doi.org/10.1007/s10237-023-01761-y