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Introgression of Oncorhynchus masou subsp. (Biwa salmon) genome into lake-run O. m. ishikawae (Amago salmon) introduced into Lake Biwa, Japan

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Abstract

An investigation to determine the extent of introgression between Biwa salmon Oncorhynchus masou subsp. and lake-run Amago salmon O. m. ishikawae from Lake Biwa was conducted using mtDNA sequence and amplified fragment length polymorphism (AFLP) analysis. Hatchery-reared Amago salmon from the Samegai Trout Farm, the main source of stock released into the inflowing rivers of Lake Biwa, were also investigated. The extent of mtDNA introgression between these two subspecies was quite low: an anomalous haplotype was detected in just 1 of 55 Biwa salmon and 2 of 63 lake-run Amago salmon. Many of the mtDNA haplotypes found in lake-run Amago salmon were common to those in hatchery-reared Amago salmon. In the AFLP analysis, the nuclear genome of Amago salmon was hardly detected in Biwa salmon, but the Biwa salmon genome comprised 13.4% of the total amplified fragments from lake-run Amago salmon, being detected in different proportions in 28 of the 63 investigated individuals of the latter subspecies. These results suggest that introgression has occurred between Biwa salmon and lake-run Amago salmon descending from hatchery-reared Amago salmon from the Samegai Trout Farm.

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Acknowledgments

We thank Drs. Masayoshi Maehata, Mark J. Grygier (Lake Biwa Museum), and Prof. Yasushi Harada (Faculty of Bioresources, Mie University) for encouragement and critical reading of the manuscript. Our special thanks go to Mr. Makoto Kobayashi (Shiga Prefectural Fishery Cooperative) for valuable information regarding egg collection and stocking of Biwa salmon. We also are grateful to the Momose Fisheries Cooperative Association and Samegai Trout Farm for providing fish for this study. This work was conducted as part of a Collaborative Research Project of the Lake Biwa Museum (Kyo 06-02).

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Authors and Affiliations

  1. Lake Biwa Museum, 1091 Oroshimo-cho, Kusatsu, Shiga, 525-0001, Japan

    Masayuki Kuwahara

  2. National Fisheries University, 2-7-1 Nagata-Honmachi, Shimonoseki, Yamaguchi, 759-6595, Japan

    Hiroshi Takahashi

  3. Shiga Prefectural Fisheries Experiment Station, 2138-3 Hassaka-cho, Hikone, Shiga, 522-0057, Japan

    Takeshi Kikko

  4. Takashima City Makino Junior High School, 601 Hirukuchi, Makino-cho, Takashima, Shiga, 520-1833, Japan

    Seiji Kurumi

  5. National Research Institute of Aquaculture, 1088 Komaki, Ueda, Nagano, 386-0031, Japan

    Kei’ichiro Iguchi

Authors
  1. Masayuki Kuwahara
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  2. Hiroshi Takahashi
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  3. Takeshi Kikko
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  4. Seiji Kurumi
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  5. Kei’ichiro Iguchi
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Correspondence to Masayuki Kuwahara.

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Kuwahara, M., Takahashi, H., Kikko, T. et al. Introgression of Oncorhynchus masou subsp. (Biwa salmon) genome into lake-run O. m. ishikawae (Amago salmon) introduced into Lake Biwa, Japan. Ichthyol Res 59, 195–201 (2012). https://doi.org/10.1007/s10228-011-0270-y

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