Abstract
The existence of an egg-laying hormone (ELH) was identified for the first time in the black tiger shrimp, Penaeus monodon, by means of immunoenzyme and immunofluorescence techniques. This was achieved using a polyclonal antibody produced against expressed recombinant ELH of the female Australian blacklip abalone, Haliotis rubra. The shrimp ELH reactive material was found to be localised within female neurosecretory tissues and the secretory tissue of the antennal gland, but was not identified in the X-organ sinus gland within the eyestalk. It was also present in the ovary, where the amount of ELH present was observed to be greatest in the period prior to spawning. These findings implied that the induction of P. monodon spawning might be involved with humoral regulation relating to ELH expression.
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Acknowledgements
We wish to thank Nigel Preston and Melony Sellar, CSIRO Marine Laboratory, for supplying specimen of live shrimp. The assistance provided by Apichart Nernsoungnern was most appreciated. We thank the Thailand Research Fund and National Center for Genetic Engineering and Biotechnology of Thailand for providing financial support.
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Liu, Z., Sobhon, P., Withyachumnarnkul, B. et al. Identification of a putative egg-laying hormone in neural and ovarian tissues of the black tiger shrimp, Penaeus monodon, using immunocytochemistry. Invert Neurosci 6, 41–46 (2006). https://doi.org/10.1007/s10158-006-0016-0
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DOI: https://doi.org/10.1007/s10158-006-0016-0