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Differential expression of miR-let7a in hair follicle cycle of Liaoning cashmere goats and identification of its targets

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Abstract

In order to improve the production and quality of Chinese cashmere, the research of hair follicle development has aroused more and more attention; the regulation mechanism of miRNA in hair follicle development has become a hot spot. A survey of transcriptome profiling screened 10 hair follicle-related miRNAs that were differentially expressed, including miR-let7a. In this study, the expression of miR-let7a was lower in anagen of hair follicle of cashmere goats than that in catagen of hair follicle of cashmere goats (p < 0.01). Results were in accordance with transcriptome data. The expression patterns of miR-let7a target genes (IGF-1R, C-myc, and FGF5) were verified by qRT-PCR, which were consistent with the results of Western blot and showed a downward trend. The dual-luciferase reporter gene system was used to verify the correlation between the expression of miR-let7a and its target genes, and it showed that miR-let7a negatively correlates with C-myc and FGF5. Present study offers new information on miRNAs and their related target genes in the regulation of hair follicle development mechanism.

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Acknowledgments

This research was supported by the Cashmere Goat Breeding Center of Liaoning province.

Funding

This work was supported by the Jilin Province Natural Science Foundation (20170101156JC), the Special Funds for Scientific Research on Public Causes (201303119), the Special Foundation for Postdoctor of China Ministry of Education (No. 20100471261), and the grants from the National Natural Science Foundation of China (NSFC) (Nos. 30800807 and 31072097).

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Author notes

  1. Tao Ma and Jianping Li contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Jilin University, Changchun, 130062, China

    Tao Ma, Qian Jiang, Sufang Wu & Qiaoling Zhang

  2. College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, 132101, China

    Jianping Li

  3. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China

    Huaizhi Jiang

Authors
  1. Tao Ma
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  2. Jianping Li
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  3. Qian Jiang
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  4. Sufang Wu
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  5. Huaizhi Jiang
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  6. Qiaoling Zhang
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This study was conducted in strict compliance with the recommendations of under the guidance of Jilin University Animal Care and Use Committee.

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The authors declare that they have no conflict of interest.

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Ma, T., Li, J., Jiang, Q. et al. Differential expression of miR-let7a in hair follicle cycle of Liaoning cashmere goats and identification of its targets. Funct Integr Genomics 18, 701–707 (2018). https://doi.org/10.1007/s10142-018-0616-x

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  • DOI: https://doi.org/10.1007/s10142-018-0616-x

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