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Expressed Sequence Tag Analysis of Kidney and Gill Tissues from Rainbow Trout (Oncorhynchus mykiss) Infected with Infectious Hematopoietic Necrosis Virus

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Abstract:

Expressed sequence tags (ESTs) were obtained from the kidney and gill tissues of rainbow trout, Oncorhynchus mykiss, infected with infectious hematopoietic necrosis virus (IHNV). The results of single-pass sequencing of ESTs from 198 clones (AU081027–AU081192) from kidney complementary DNA and 45 clones (AU081193–AU081236) from gill cDNA are reported herein. Sequences of the cDNA clones were compared with sequences in the GenBank database. Fourteen clones (20%) appeared to be completely unknown and may represent newly described genes, whereas 158 clones (80%) were identified on the basis of matches to sequences in the database. Three of the unidentified sequences were isolated from both the kidney and the gill cDNA libraries. However, no sequences were identical between kidney and gill clones.

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Authors and Affiliations

  1. United Graduate School of Agricultural Science, Kagoshima University, Korimoto, 1-21-24, Kagoshima 890-0065, Japan, , , , , , JP

    Tomoya Kono

  2. Faculty of Agriculture, Miyazaki University, Miyazaki 889-21, Japan, , , , , , JP

    Masahiro Sakai

  3. Clear Springs Foods Inc., P.O. Box 712, Buhl, ID 83316, U.S.A., , , , , , US

    Scott E. LaPatra

Authors
  1. Tomoya Kono
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  2. Masahiro Sakai
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  3. Scott E. LaPatra
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Received December 7, 1999; accepted April 28, 2000.

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Kono, T., Sakai, M. & LaPatra, S. Expressed Sequence Tag Analysis of Kidney and Gill Tissues from Rainbow Trout (Oncorhynchus mykiss) Infected with Infectious Hematopoietic Necrosis Virus. Mar. Biotechnol. 2, 493–498 (2000). https://doi.org/10.1007/s101260000032

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  • DOI: https://doi.org/10.1007/s101260000032

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