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Physiological and Transcriptome Analysis Reveal the Regulation Mechanism Underlying the Muscle Quality Effect of Dietary Schisandra chinensis in Triploid Crucian Carp (Carassius auratus)

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Abstract

Schisandra chinensis (sc) is generally demonstrated to improve antioxidant and immune functions in mammal. The present study through physiological and transcriptome analysis revealed alterations in muscle metabolisms of triploid crucian carp (Carassius auratus) cultured at different concentrations of S. chinensis diets (sc0, sc0.125%, sc0.25%, sc0.5%, sc1%, sc2%) after 8 weeks. The serum antioxidant enzyme activities analysis showed that dietary S. chinensis could reduce oxidative stress and increase organismic antioxidant capacity. Meanwhile, the detected results of muscle components presented that the amino acids and two flavor nucleotides of GMP and IMP significantly elevated while muscle crude lipid significantly reduced in S. chinensis feeding groups. In addition, springiness, chewiness, and fiber density in S. chinensis feeding groups muscle were significantly upregulated while muscle fiber diameter and area showed an opposite trend. By comparative transcriptome analysis of the muscles, functional enrichments of differentially expressed genes showed that multiple terms were related to purine metabolism, glycerolipid metabolism, regulation of actin cytoskeleton, and peroxisome. Finally, some key hub genes such as egln, gst, ggct, su1b, pi3kr4, myh9, lpl, gcdh, mylk, and col4a were identified by weighted gene co-expression network analysis. Taken together, our findings facilitate the understanding of the molecular basis underlying the muscle quality effect of dietary S. chinensis in triploid crucian carp, which provides valuable insights into the nutritional strategies of the aquaculture industry.

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Availability of Data and Materials

Data will be made available on request. RNA sequencing raw reads have been deposited in NCBI’s Sequence Read Archive (SRA) under BioProject accession number PRJNA999921.

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Funding

This research was supported by the grants from the National Natural Science Foundation of China (Grant No. U19A2041); the Project of Scientific Research of Hunan Provincial Education Department, China (Grant No.20B058); and the Natural Science Foundation of Changsha, Hunan Province, China (Grant No. kq2208427).

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Authors and Affiliations

  1. Hunan Provincial Key Laboratory of Nutrition and Quality Control of Aquatic Animals, Department of Biological and Chemical Engineering, Changsha University, Changsha, 410022, China

    Anli Zuo, Yonghua Zhou, Yuxian Chen, Sipu Liu, Yuyao Lu, Yingjie Li, Shenping Cao & Zhen Liu

  2. College of Life Sciences, Hunan Normal University, Changsha, 410081, China

    Anli Zuo

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  1. Anli Zuo
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  2. Yonghua Zhou
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Contributions

ZL: experimental design and coordination, manuscript revision and funding acquisition. AZ: completion of the experiment, data analysis, and manuscript drafting. YZ: data curation and funding acquisition. YC: investigation. SL: investigation. YL: investigation. YL: investigation. SC: resources and supervision.

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Correspondence to Zhen Liu.

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The triploid crucian carp is neither an endangered nor protected species. All experiments in this study were conducted according to national and institutional guidelines.

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Zuo, A., Zhou, Y., Chen, Y. et al. Physiological and Transcriptome Analysis Reveal the Regulation Mechanism Underlying the Muscle Quality Effect of Dietary Schisandra chinensis in Triploid Crucian Carp (Carassius auratus). Mar Biotechnol 25, 1191–1207 (2023). https://doi.org/10.1007/s10126-023-10270-z

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