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Genome Scan for Genomic Regions and Genes Associated with Growth Trait in Pacific White Shrimp Litopeneaus vannamei

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Abstract

The Pacific white shrimp Litopeneaus vannmei (L. vannmei) is a predominant aquaculture shrimp species worldwide, and it is considered as the aquaculture species with the highest single output value. Advances in selective breeding have accelerated the development of L. vannmei aquaculture. Recently, the genome-wide association studies (GWAS) have been applied in aquaculture animals and markers associated with economic traits were identified. In this study, we focused on the growth trait of L. vannamei and performed GWAS to identify SNPs or genes associated with growth. Genomic regions in linkage group 7, 27, 33, and 38 were identified to be associated with body weight and body length of the shrimp. Further, candidate gene association analysis was performed in two independent populations and the result demonstrated that the SNPs in the genes protein kinase C delta type and ras-related protein Rap-2a were significantly associated with the growth trait of L. vannamei. This study showed that GWAS analysis is an efficient approach for screening trait-related markers or genes. The genomic regions and genes identified in this study are essential for further fine mapping of growth-related genes. The identified markers will provide useful information for marker-assisted selection in L. vannamei.

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Funding

This work is supported by the National Key R&D Program of China (2018YFD0901301, 2018YFD0900103), National Natural Science Foundation of China (31830100), and China Agriculture Research System-48.

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  1. Yang Yu and Quanchao Wang contributed equally to this manuscript.

Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Yang Yu, Quanchao Wang, Qian Zhang, Zheng Luo, Yue Wang, Xiaojun Zhang, Jianhai Xiang & Fuhua Li

  2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China

    Yang Yu, Xiaojun Zhang, Jianhai Xiang & Fuhua Li

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Qian Zhang, Zheng Luo & Yue Wang

  4. Hainan Grand Suntop Ocean Breeding Co., Ltd, Wenchang, 571300, China

    Hao Huang

  5. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China

    Jianhai Xiang & Fuhua Li

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  1. Yang Yu
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Yu, Y., Wang, Q., Zhang, Q. et al. Genome Scan for Genomic Regions and Genes Associated with Growth Trait in Pacific White Shrimp Litopeneaus vannamei. Mar Biotechnol 21, 374–383 (2019). https://doi.org/10.1007/s10126-019-09887-w

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