Abstract
Preliminary genetic linkage maps were constructed for the Pacific abalone (Haliotis discus hannai Ino) using amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA (RAPD), and microsatellite markers segregating in a F1 family. Nine microsatellite loci, 41 RAPD, and 2688 AFLP markers were genotyped in the parents and 86 progeny of the mapping family. Among the 2738 markers, 384 (including 365 AFLP markers, 10 RAPD markers, and 9 microsatellite loci) were polymorphic and segregated in one or both parents: 241 in the female and 146 in the male. The majority of these markers, 232 in the female and 134 in the male, segregated according to the expected 1:1 Mendelian ratio (α = 0.05). Two genetic linkage maps were constructed using markers segregating in the female or the male parent. The female framework map consisted of 119 markers in 22 linkage groups, covering 1773.6 cM with an average intermarker space of 18.3 cM. The male framework map contained 94 markers in 19 linkage groups, spanning 1365.9 cM with an average intermarker space of 18.2 cM. The sex determination locus was mapped to the male map but not to the female map, suggesting a XY-male determination mechanism. Distorted markers showing excess of homozygotes were mapped in clusters, probably because of their linkage to a gene that is incompatible between two parental populations.
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Acknowledgments
We thank Dr. Li Li for reviewing this paper, Jie Xiao for RAPD analysis. This research was supported by National High-Technology R & D Program of China (2003AA603023 and 2004AA626070) and National Science Foundation of China (30371117 and 39825121).
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Liu, X., Liu, X., Guo, X. et al. A Preliminary Genetic Linkage Map of the Pacific Abalone Haliotis discus hannai Ino. Mar Biotechnol 8, 386–397 (2006). https://doi.org/10.1007/s10126-005-6133-7
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DOI: https://doi.org/10.1007/s10126-005-6133-7