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Use of Chitosan Membrane from the Carapace of the Soldier Crab Mictyris brevidactylus for Biosensor Construction

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Abstract

Glucose oxidase (EC 1.3.4.3) was immobilized on chitosan membrane (<0.1 mm in thickness) prepared from the carapace of the soldier crab Mictyris brevidactylus. A glucose electrode was constructed by covering a platinum electrode (2.0 mm in diameter) with the enzyme membrane. The enzyme electrode sensed glucose amperometrically (1.0 µA/mM, with linear range up to 0.5 mM, r = 0.999) when positively imposed with 0.6 V against an Ag/AgCl reference electrode. The glucose biosensor was sensitive (<0.1 µM, S/N > 3), reproducible (CV for 55 µM glucose <3%, n = 5), reagentless, and durable for months.

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Authors and Affiliations

  1. Department of Bio-Industrial Mechatronics Engineering, College of Agriculture, National Taiwan University, 1, Roosevelt Road, Sec. 4, Taipei, Taiwan 106, Republic of China

    Bo-Chuan Hsieh, Tzong-Jih Cheng, Tzu-Yu Wang & Richie L. C. Chen

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  1. Bo-Chuan Hsieh
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  2. Tzong-Jih Cheng
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  3. Tzu-Yu Wang
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  4. Richie L. C. Chen
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Correspondence to Richie L. C. Chen.

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Hsieh, BC., Cheng, TJ., Wang, TY. et al. Use of Chitosan Membrane from the Carapace of the Soldier Crab Mictyris brevidactylus for Biosensor Construction . Mar. Biotechnol. 5, 119–125 (2003). https://doi.org/10.1007/s10126-002-0094-x

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  • DOI: https://doi.org/10.1007/s10126-002-0094-x

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