Abstract
Ophiocordyceps sinensis is an entomogenous fungus, and its wildlife resource is very insufficient, as it is widely traded as a natural health product. The artificial culture of O. sinensis is a remarkably effective progress in addressing the problem. Adding microorganisms may improve the process of artificial culture. To analyse the composition and function of the microbial community, high-throughput sequencing was used to explore the microbial community inhabiting wild and artificial O. sinensis and surrounding soil. Significant differences in the microbial communities across groups were revealed by the PCoA analysis. There were 51 fungal and 598 bacterial operational taxonomic units only being assigned to the fruiting bodies of wild O. sinensis (Wf) by the Venn diagram. From the LEfSe analysis, 39 fungal taxa and 75 bacterial taxa were enriched in Wf. Enzymes that were highly abundant in the core fungi were involved in physiological metabolic processes. Metabolic pathways were dominated in the core bacteria, followed by environmental information processing. The core microorganisms, with the marked differences between Wf and the other three groups, were essential for wild O. sinensis. Functional analysis verified their involvement in the growth, development, and infection of O. sinensis. These core microorganisms may be a valuable resource for the artificial culture of O. sinensis.
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Data availability
The datasets generated during and/or analysed during the current study are available in the NCBI Sequence Read Archive (SRA) database, accession number: SRR17549241–SRR17549280.
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Acknowledgements
We appreciate Dr. Shabana Bibi for the English language editing of the whole manuscript.
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This work was supported by the National Natural Science Foundation of China (31870017) and the Ministry of Ecology and Environment of China (2019HJ2096001006).
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All authors contributed to the study conception and design. Material preparation was performed by Canming Zhang and Run Luo. Data collection and analysis were performed by Tao Sun, Weiqiu Zou, and Chengpeng Li. The first draft of the manuscript was written by Tao Sun, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Sun, T., Zou, W., Luo, R. et al. Compositional and functional diversities of core microbial communities in wild and artificial Ophiocordyceps sinensis. Int Microbiol 26, 791–806 (2023). https://doi.org/10.1007/s10123-023-00333-5
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DOI: https://doi.org/10.1007/s10123-023-00333-5