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Expression and degranulation of mast cells in laser photobiomodulated mucositis chemo-induced: pilot study in hamsters

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Abstract

This study aimed to evaluate the influence of laser photobiomodulation on the expression and degranulation of mast cells in chemo-induced oral mucositis (OM) lesions in hamsters. Twelve adult male Syrian hamsters (Mesocricetus auratus), golden lineage, were submitted to OM induction. They were divided into three groups: control—OM without treatment (C), OM treated with red laser (RL), OM treated with infrared laser (IL) and analyzed in the experimental time of 7 days. Three and 4 days after the intraperitoneal injection of the chemotherapy drug fluorouracil, the OM lesions were induced by making grooves in the right cheek pouch. Immediately after chemoinduction, the hamsters were submitted to photobiomodulation every 48 h for 7 days. The specimens were processed and stained using the hematoxylin-eosin and toluidine blue techniques. There was a predominance of mild chronic inflammation in the experimental groups and a greater persistence of neutrophils in the control group (C), although not statistically significant. The group irradiated with red laser (RL) had the highest mean mast cell expression (38.28 ± 19.05) (p < 0.001). As for the degranulation activity in mast cells, the control group (C) showed a greater number of fields with more than 50% of degranulated cells, presenting statistical significance when comparing it with the RL (p < 0.009) and IL (p = 0.036) group. It can be concluded that photobiomodulation, at both wavelengths, decreased mast cell degranulation, accelerating the inflammatory process. The use of infrared laser provided, in addition to less degranulation, the quantitative reduction of mast cells.

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Acknowledgements

This work received support from the Coordination for the Improvement of Higher Education Personnel (CAPES), which granted a master’s scholarship to the author Naiadja de Santana Cerqueira.

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Authors and Affiliations

  1. Postgraduate Program in Dentistry and Health, Faculty of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil

    Naiadja de Santana Cerqueira

  2. Salvador, Brazil

    Lívia Andrade Vitória

  3. Department of Oral Diagnosis, UNIFTC University Center, Salvador, Bahia, Brazil

    Viviane Palmeira da Silva

  4. Department of Bioregulation, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil

    Tânia Tavares Rodriguez

  5. Department of Social and Pediatric Dentistry, Faculty of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil

    Maria Cristina Teixeira Cangussu

  6. Department of Propaedeutics and Integrated Clinic, Faculty of Dentistry, Federal University of Bahia, Araújo Pinho Avenue, no. 62, Canela, 9th floor, Salvador, Bahia, 40301-155, Brazil

    Luciana Maria Pedreira Ramalho

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  1. Naiadja de Santana Cerqueira
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  2. Lívia Andrade Vitória
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Correspondence to Luciana Maria Pedreira Ramalho.

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de Santana Cerqueira, N., Vitória, L.A., da Silva, V.P. et al. Expression and degranulation of mast cells in laser photobiomodulated mucositis chemo-induced: pilot study in hamsters. Lasers Med Sci 38, 31 (2023). https://doi.org/10.1007/s10103-022-03698-7

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