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Low-level laser therapy prevents muscle oxidative stress in rats subjected to high-intensity resistance exercise in a dose-dependent manner

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Abstract

High-intensity resistance exercise (RE) increases oxidative stress leading to deleterious effects on muscle performance and recovery. The aim of this study was to assess the effect of applying low-level laser therapy (LLLT) prior to a RE session on muscle oxidative stress and to determine the possible influence of the dosimetric parameters. Female Wistar rats were assigned to non-LLLT (Ctr: non-exercised control; RNI: RE) or LLLT groups subjected to RE (radiant energy: 4 J, 8 J, and 12 J, respectively). RE consisted of four maximum load climbs. An 830-nm DMC Lase Photon III was used to irradiate three points in gastrocnemius muscles (two limbs) before exercise. Animals were euthanized after 60 min after the end of the exercise, and muscle tissue was removed for analysis of oxidative stress markers. All doses resulted in the prevention of increased lipoperoxidation; however, LLLT prevented protein oxidation only in rats that were pretreated with 8 J and 12 J of energy by LLLT. RE and LLLT did not change catalase activity. However, RE resulted in lower superoxide dismutase activity, and the opposite was observed in the LLLT group. These data indicate that LLLT prior to RE can prevent muscle oxidative stress. This study is the first to evaluate the impact of dosimetric LLLT parameters on the oxidative stress induced by RE, wherein both 8 J and 12 J of energy afforded significant protection.

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Acknowledgments

The authors are grateful to Editage for English language editing.

Funding

This work was supported by the São Paulo Research Foundation (FAPESP) [grant 2018/06865–7] and Brazilian National Council for Scientific and Technological Development (CNPq) [grant 305,527/2017–7]. Funding sources were not involved in the study design, collection, analysis, or interpretation of data. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding sources.

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Authors and Affiliations

  1. Biophotonics Applied to Health Science, Nove de Julho University, São Paulo, SP, Brazil

    Simone Silva dos Santos, Barbara Sampaio Dias Martins Mansano, Paulo Tarso Camillo de Carvalho & Andrey Jorge Serra

  2. Department of Medicine, Cardiology Division, Federal University of São Paulo, São Paulo,, SP, Brazil

    Helenita Antonia de Oliveira, Ednei Luiz Antonio, Ighor Luiz Azevedo Teixeira, Flávio André Silva, Paulo José Ferreira Tucci & Andrey Jorge Serra

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  1. Simone Silva dos Santos
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  2. Helenita Antonia de Oliveira
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Correspondence to Andrey Jorge Serra.

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The study was approved by the Institutional Research Ethics Committee of the Federal University of São Paulo, São Paulo – SP, Brazil (process no 8868250615).

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dos Santos, S.S., de Oliveira, H.A., Antonio, E.L. et al. Low-level laser therapy prevents muscle oxidative stress in rats subjected to high-intensity resistance exercise in a dose-dependent manner. Lasers Med Sci 35, 1689–1694 (2020). https://doi.org/10.1007/s10103-020-02951-1

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