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Anti-inflammatory effect of low-intensity laser on the healing of third-degree burn wounds in rats

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Abstract

Third-degree burn wounds are considered severe injuries because they destroy all the skin layers and may affect subcutaneous tissues, fasciae, muscles, and bones. To favor the healing process of the injured tissues, it is very useful to diminish the occurrence of the inflammatory process. The present study was aimed at comparing the effect of different energetic densities of AlGaInP laser on the inflammatory process and in the healing of third-degree burn wounds in Wistar rats. This study was approved by the Ethics Committee, in which 36 adult male rats were selected and suffered the induction of third-degree burn injury. These rats were divided as follows: group 1—control (treated with silver sulfadiazine), group 2—received energy density of 3 J/cm2, and group 3—received energy density of 6 J/cm2. All animals daily received an occlusive bandage with silver sulfadiazine and 8 % papain. The laser therapy was performed alternatively three times a week. The animals were evaluated on the 3rd, 7th, 14th, and 21st days after the initial lesion and euthanized for the macroscopic, histologic, and morphometric analysis. A higher production of collagen was observed at 7 days and a greater re-epithelialization at 21 days in group 3 (6 J/cm2). Furthermore, the latter when compared to the other groups presented macroscopically a better aspect of the scar at 21 days with more granulation tissue and fibrosis. We conclude that the AlGaInP laser used in dosages of 3 and 6 J/cm2 favors the healing of third-degree burn wounds induced in rats.

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References

  1. Vale ECS (2005) Primeiro atendimento em queimaduras: a abordagem do dermatologista. An Bras Dermatol 80:9–19

    Google Scholar 

  2. Lima Júnior EM, Novaes FN, Piccolo NS, Serra MC (2008) Tratado de queimaduras no paciente agudo, 2nd edn. Atheneu, São Paulo

    Google Scholar 

  3. Montes SF, Barbosa MH, Sousa Neto AL (2011) Aspectos clínicos e epidemiológicos de pacientes queimados internados em um Hospital de Ensino. Rev Esc Enferm USP 45:369–373

    Article  PubMed  Google Scholar 

  4. Vendrusculo TM, Balieiro CRB, Echevarría-Guanilo ME, Farina Junior JA, Rossi LA (2010) Queimaduras em ambiente doméstico: características e circunstâncias do acidente. Rev Lat Am Enfermagem 18:444–451

    Article  PubMed  Google Scholar 

  5. Blanes L (2011) Tratamento de feridas. Cirurgia vascular: guia ilustrado. São Paulo [on line]. http://www.bapbaptista.com. Accessed 01 Nov 2011

  6. Fernandes LRA (2005) Fisiologia da cicatrização–feridas e curativos. [on line]. http://www.unimes.br/aulas/MEDICINA/Aulas2005/1ano/Procedimentos_basicos_em_medicina/feridas_e_curativos.html. Accessed 01 Nov 2010

  7. Azevedo AMZ, Machado MJ, Chassot GC (2002) Assistência de enfermagem nos pacientes queimados com curativo aberto na unidade de internação. Mom Perspec Saúde 15:1–7

    Google Scholar 

  8. Santucci SG, Gobara S, Santos CR, Fontana C, Levin AS (2003) Infections in a burn intensive care unit: experience of 7 years. J Hosp Infect 53:6–13

    Article  PubMed  CAS  Google Scholar 

  9. Bourguignon-Filho AM, Feitosa ACR, Beltrão GC, Pagnoncelli RM (2005) Utilização do laser de baixa intensidade no processo de cicatrização tecidual. Rev Port Estomatol Cir Maxilofac 46:37–43

    Google Scholar 

  10. Veçoso MC (1993) Laser em fisioterapia. Lovise, São Paulo

    Google Scholar 

  11. Bayat M, Vasheghani M, Razavi N (2008) Effects of low-level laser therapy on mast cell number and degranulation in third-degree burns of rats. JRRD 45:931–938

    Article  Google Scholar 

  12. Pereira RM (2005) Efeitos dos lasers de baixa potência em três diferentes comprimentos de onda no processo de cicatrização de queimaduras de 3° grau. Thesis. Universidade Vale do Paraíba

  13. Schlager K, Oehler K, Huebner M, Schmuth L, Spoetl L (2000) Healing of burns after treatment with 670-nanometer low-power laser light. J Dermatol Surg Austria 105:1635–1639

    CAS  Google Scholar 

  14. Santuzzi CH, Buss HF, Pedrosa DF, Freire MOV, Nogueira BV, Goncalves WLS (2011) Uso combinado da laserterapia de baixa potência e da inibição da ciclooxigenase-2 na reepitelização de ferida incisional em pele de camundongos: um estudo pré-clínico. An Bras Dermatol 86:278–283

    Article  PubMed  Google Scholar 

  15. Júnior AMR, Oliveira RG, Farias RE, Andrade LCF, Aarestrup FM (2006) Modulação da proliferação fibroblástica e da resposta inflamatória pela terapia a laser de baixa intensidade no processo de reparo tecidual. An Bras Dermatol 81:150–156

    Google Scholar 

  16. Reddy GK (2004) Photobiological basis and clinical role of low-intensity lasers in biology and medicine. J Clin Laser Med Surg 22:141–150

    Article  PubMed  Google Scholar 

  17. Posten W, Wrone DA, Dover JS, Arndt KA, Silapunt S, Alam M (2005) Low-level laser therapy for wound healing: mechanism and efficacy. Dermatol Surg 31:334–340

    Article  PubMed  CAS  Google Scholar 

  18. Maiya GA, Kumar P, Rao L (2005) Effects of low intensity helium-neon (He-Ne) laser irradiation on diabetic wound healing dynamics. Photomed Laser Surg 3:187–190

    Article  Google Scholar 

  19. Guirro ECO, Guirro RRJ (2004) Fisioterapia dermato funcional: fundamentos, recursos, patologias, 3rd edn. Manole, São Paulo

    Google Scholar 

  20. Lange F, Kroth A, Steffani JA, Lorencetti N (2003) Influência da laserterapia no processo cicatricial de queimaduras de terceiro grau. Fisioter Bras 4:335–340

    Google Scholar 

  21. Gomes DR, Serra MC, Pellon MA (1995) Queimaduras. Revinter Ltda, Rio de Janeiro

    Google Scholar 

  22. Mello PB, Sampedro RMF, Piccinini AM (2007) Efeitos do laser HeNe e do modo de aplicação no processo de cicatrização de queimaduras em ratos. Fisioterapia Pesquisa 14:6–13

    Google Scholar 

  23. Low J, Reed A (2001) Eletroterapia explicada princípios e prática. Manole, São Paulo

    Google Scholar 

  24. Oliveira PC, Meireles GCS, Santos NR, Carvalho CM, Souza APC, Santos JN, Pinheiro ALB (2008) The use of light photobiomodulation on the treatment of second-degree burns: a histological study of a rodent model. Photomed Laser Surg 26:289–299

    Article  PubMed  Google Scholar 

  25. Meirelles GCS (2005) Análise comparativa do efeito dos lasers GaAlAs de 660 nm e 780 nm na cicatrização de úlceras por queimadura em dorso de ratos diabéticos e não-diabéticos: estudo histológico. Thesis. Universidade Federal da Bahia

  26. Gál P, Vidinsky’ B, Toporcer T, Mokrý M, Mozes S, Longauer F, Sabo J (2006) Histological assessment of the effect of laser irradiation on skin wound healing in rats. Photomed Laser Surg 24:480–488

    Article  PubMed  Google Scholar 

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Acknowledgments

The authors would like to thank Sharon Lois Vinaud for the English review. The authors would also like to thank the Burning Wounds Hospital of Goiania (Hospital de Queimaduras de Goiania) and Eduardo Di Oliveira Pires from the Núcleo Integrado de Reabilitação e Educação for providing the AlGaInP laser for this study, and Dr. Nelson Sarto Piccolo from the Nelson Piccolo Institute and Farmácia Artesanal for providing the papain.

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Authors and Affiliations

  1. Biological Sciences Institute, Federal University of Goias (UFG), Campus II—Samambaia, saída para Nerópolis—Km 13, Goiânia, GO, 74001-970, Brazil

    Juliana Medeiros de Moraes & Diego Eterno de Oliveira Mendonça

  2. Tropical Pathology and Public Health Institute, UFG, rua 235, s/n, Setor Universitario, Goiânia, GO, 74650-050, Brazil

    Vânia Beatriz L. Moura, Milton Adriano Pelli Oliveira, Marina Clare Vinaud & Ruy de Souza Lino Jr.

  3. Faculty of Physical Education and Physiotherapy of Goias, UEG, Avenida Anhanguera, n° 1420, Setor Vila Nova, Goiânia, GO, Brazil

    Cristina Lopes Afonso

  4. Nursing Faculty, UFG, Rua 227 Qd 68, S/N—Setor Leste Universitário, Goiânia, GO, 74605-080, Brazil

    Maria Márcia Bachion

Authors
  1. Juliana Medeiros de Moraes
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  2. Diego Eterno de Oliveira Mendonça
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  3. Vânia Beatriz L. Moura
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  4. Milton Adriano Pelli Oliveira
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  5. Cristina Lopes Afonso
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  6. Marina Clare Vinaud
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  7. Maria Márcia Bachion
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  8. Ruy de Souza Lino Jr.
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Correspondence to Marina Clare Vinaud.

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de Moraes, J.M., Eterno de Oliveira Mendonça, D., Moura, V.B.L. et al. Anti-inflammatory effect of low-intensity laser on the healing of third-degree burn wounds in rats. Lasers Med Sci 28, 1169–1176 (2013). https://doi.org/10.1007/s10103-012-1213-1

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  • DOI: https://doi.org/10.1007/s10103-012-1213-1

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